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Stress induced morphogenic response in plant root system
Otradovcová, Michala ; Soukup, Aleš (advisor) ; Vaňková, Radomíra (referee)
The root system architecture adjustment is one of the possible plant reactions to stress effects of environmental conditions. The shape of root system is jointly formed by the root apical meristem activity, the rate of elongation of root cells and by the degree of formation, developement and organization of secondary and adventitious roots. This paper aims to describe basic mechanisms of the root system developement and outline the morphological and regulatory changes occuring during development while being affected by selected stress factors.
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File of educational materials to support the teaching of mathematics
Hanzal, Petr ; Battistová, Eva (advisor) ; Štípek, Jiří (referee)
The aim of this bachelor thesis is to suggest and create the collection of solved exercises from mathematic on a theme quadratic, absolute-value equations and equations involving square roots. It is intended for the students of the fifth grade of the eight-year grammar school and for the students of the first grade of the four-year grammar school or secondary school. Thanks to the solved exercises it is possible for students to get to know the methods for solving given types of quadratics more deeply.
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Tissue-specific knockout of starch synthesis in columella cells of Arabidopsis thaliana and gravitropic response
Bogdan, Michal ; Fendrych, Matyáš (advisor) ; Retzer, Katarzyna (referee)
Since the studies of plant gravitropism by Charles Darwin, the identity of specific sensors of gravity in plants has been uncertain. To this date, statoliths - starch granules in the root tips - are considered to play a key role in gravity sensing. The role of statoliths as organelles that mediate the gravity sensing ability of plant roots is based on research that uses plants which have severely impaired ability to synthesize starch in general or have their cells that contain statoliths removed or damaged. This represents methodical imperfections that give rise to alternative explanations, like disturbed auxin flow due to heavy damage to the root tip or unknown involvement of starch from other parts of the plant in gravity perception. Thanks to advances in the field of CRISPR/Cas9 technology, we are now able to produce tissue-specific mutants that might help with clarification of whether starch granules in the root tip are involved in sensing gravity and if so, how significant is this involvement. This diploma thesis aimed to answer these questions by adapting the tissue-specific CRISPR/Cas9 system and using it for the creation of mutants that are starchless specifically in the columella cells. Using this approach, we generated one tissue non-specific mutant line and three tissue-specific mutant...
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Visualization of root apoplastic pH in plants
Wernerová, Daša ; Fendrych, Matyáš (advisor) ; Paris, Nadine (referee)
Plant oriented movements, or tropisms allow the plant to actively respond to environmental stimuli to get more light, better access to nutrients and to grow roots deeper into the soil. Gravitropism drives the growth of roots along the gravity vector. Perception of gravity is triggered by the sedimentation of statoliths in columella root cap, but the exact signalling pathway behind this process is not known. Perception of gravity results in an unequal redistribution of the phytohormone auxin in the outer cell layers which leads to different rate of growth on the root's upper and lower side and bending of the root. The changes in auxin redistribution are accompanied by changes in apoplastic pH. Knowing an exact pattern of these pH changes could shed light on the mechanisms laying behind the gravitropic response pathway. While microelectrodes can be used to measure pH precisely, they are not suitable for the long-term imaging of growing roots. In the past few years, several pH sensitive dyes and genetically encoded sensors emerged. These can be used for long-term live in vivo imaging of pH changes in growing roots. In this thesis, I analysed the performance of several published pH sensitive genetically encoded sensors and available dyes in the roots of Arabidopsis thaliana. I observed that dyes varied...
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Use of kinematic-constrained decays in the alignment of the Belle II detector.
Kaplan, Martin ; Kodyš, Peter (advisor) ; Kvasnička, Peter (referee)
This thesis is a Monte Carlo study of several distinct samples and some of their combinations for track-based alignment of tracking detectors at the Belle II experiment. Simultaneous alignment of drift chamber layers, vertex detector sensors including their deformation parameters and vertex detector half-shells is performed using a set of linear constraints. Samples of muon tracks from cosmic rays, muon pairs from the e+ e− → µ+ µ− process with and without the inter- action point vertex constraint and muon pairs from the kinematic-constrained J/Ψ → µ+ µ− decay are studied. Performance of certain combinations of sam- ples in the alignment procedure of the tracking detectors is analyzed. One combination of samples is identified as a suitable sample for the alignment of the real detector, which started its operation in spring 2019 and thus the results of this thesis present a first comprehensive analysis of systematic and statistical errors associated with a realistic scenario of the Belle II detector. 1
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Tests of Semiconductor Detectors in Prague Laboratory
Krásny, Peter ; Kodyš, Peter (advisor) ; Carli, Ina (referee)
This thesis is focused on the usage of silicon semiconductor detectors in ATLAS experiment and automation of safe cooling and heating processes needed during the tests performed on detectors at a given tem- perature. The tests take place in the freezer environment with the place of interest where the detector is to be placed required to stay dry throughout the entire test. This is secured by monitoring the temperature and rela- tive humidity of the environment using Interlock device with sensors and by automatic calculation of the dew point temperature without any supply of nitrogen commonly used in other set ups of the tests. Both cooling and heating processes are being prepared to be fully automatized, reducing the amount of manual handling required to minimum which helps to maintain the conditions and safe run of the test. 1
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Technology for growing Leuzey Saflor (Leuzea Carthamoids DC) and its application
FEJO, Eva
Maralí kořen or parcha saflorová is very easy-growing plant from Asie, specifically from the area of Sibir. It has a specific form resembling thistle. It is very durable plant which can be grown in almost all kinds of climate conditions. Thanks to its origin it has wide range of utilization as well. Its use has been know for many years and it was mainly used in the place of origin. In the Czech Republic parcha is not so common because it came several years ago. However, it has very positive impacts on curing even serious illnesses, central nervous system and libido.
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Playful teaching methods for morphemic analysis of words in the first grade of primary schools
LÍSKOVCOVÁ, Lucie
This diploma thesis deals with playful methods of word-construction teaching at lower primary schools. The practical part of the thesis deals with terms connected to this subject and terms student come across at lower primary schools. The following terms will be defined: prefix, root, suffix, ending, related words, base word, base. A part of the theoretical part consists of a summary of this subject meant for Grades 3 - 5 in textbooks by different publishers. The practical part contains a number of playful exercises and activities that that may be used even in acquiring these learning topics.
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Stress induced morphogenic response in plant root system
Otradovcová, Michala ; Soukup, Aleš (advisor) ; Vaňková, Radomíra (referee)
The root system architecture adjustment is one of the possible plant reactions to stress effects of environmental conditions. The shape of root system is jointly formed by the root apical meristem activity, the rate of elongation of root cells and by the degree of formation, developement and organization of secondary and adventitious roots. This paper aims to describe basic mechanisms of the root system developement and outline the morphological and regulatory changes occuring during development while being affected by selected stress factors.
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Cellular localization and functional characterization of TTL proteins of Arabidopsis
Schier, Jakub ; Soukup, Aleš (advisor) ; Schwarzerová, Kateřina (referee)
This thesis focuses on the study of TTL protein family in the model species Arabidopsis thaliana. It sums up available published data on this so far poorly studied genes and presents newly gained experimental data. Main emphasis is given to TTLs cellular localization in possible relationship to their physiological function. Presented thesis comprises of in silico analysis of TTL proteins, but also the creation and microscopy analysis of TTL-mRuby2 and TTL-GFP fusion proteins in transient and permanent transformants. Powered by TCPDF (www.tcpdf.org)
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