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Chemokines of epithelial cells in bronchial asthma
Volfová, Dominika ; Stříž, Ilja (advisor) ; Holáň, Vladimír (referee)
Asthma is an allergic disease caused by adverse reactions to harmless antigens (allergens) and is characterized by the recruitment of eosinophils, Th2 lymphocytes, mast cells and neutrophils into the tissue site of inflammation, to the lungs. This accumulation of leukocytes is mediated by the generation of chemotactic cytokines (chemokines). Chemokines are low molecular weight proteins, functioning by binding to specific receptors on the cell surface. Binding of chemokines and their receptors is highly promiscuous and subsequent activation of effector cells is very heterogeneous, which can often complicate research in this area. However, chemokines and their receptors are important potential therapeutic targets in allergic diseases including asthma, mainly because of their central role in cell activation and inflammation. Chemokines are secreted by cells of the immune system and cells of various tissues of the body. Recently, attention turns to the role of epithelial cells in the pathogenesis of asthma. Bronchial epithelial cells stimulated by antigens produce cytokines and defense molecules used for the amplification of inflammatory processes and regulate the aktivity of effector cells. Impaired cytokine regulation may lead to the development of various lung diseases including asthma. This work...
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Immunomodulatory effects of macrolide antibiotics
Zemánková, Jana ; Stříž, Ilja (advisor) ; Krulová, Magdaléna (referee)
Macrolide antibiotics are well known not only for their antibacterial properties, but also for their recently discovered anti-inflammatory properties. They are able to significantly suppress destructive and in many cases life-threatening inflammation, an effect which is desired especially in chronic inflammatory diseases. The principle which their act is the modulation of the various components of the immune system. These effects are called "immunomodulatory" and can also include the effect on epithelial cells and their secretory activity, as well as the effect on pathogens which can colonize the airways and contibute to pathogenesis and the emergence of the chronic inflammatory respiratory diseases. This thesis summarizes the most important known mechanisms, by which macrolide antibiotics exert these immunomodulatory effects, and also notes examples of diseases whose treatment is the most clinically significant. Macrolide antibiotics posessing these uniqe anti-inflammatory properties are well tolerated and severe side-effects are rare. However, the most serious risk is the emergence of resistance and that is the main reason why this treatment can not be recommended without reservation. It is up to each doctor to consider the risks and benefits of the treatment in each individual patient.
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Animal Models of Intrauterine Infections
Schuma, Marek ; Šplíchalová, Alla (advisor) ; Seifertová, Eva (referee)
In mammals, embryonic and fetal development takes place in the mother's uterus under sterile conditions. The embryo, and later the fetus, is connected to the placenta by the umbilical cord and floats in amniotic fluid in the amniotic cavity lined by the amniotic membrane. Preterm birth is associated with decreased weight of newborns and increased morbidity and mortality. Intrauterine infections are one of the leading causes of preterm delivery. Various etiological agents, such as viruses, bacteria, yeasts and protozoa, can cause these infections. These infections induce expression of inflammatory mediators, which participate in triggering of preterm birth. One of these mediators are cytokines. The bachelor thesis is based on a literature review dealing with animal models of prenatal infections, which are exploited in the study of regulatory mechanisms of preterm births or eventually in the study of experimental manipulation of these mechanisms. Various infectious agents and their parts, such as LPS, and the routes of administration are compared. In addition, the advantages and disadvantages of different animal models are discussed with respect to the type of placentation, duration of gestation, animal weight and litter size. The thesis contains the section dealing with experience with a pig model...
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Stress of endoplasmic reticulum and its role for the development of adipose tissue inflammation
Zemánková, Kateřina ; Rossmeislová, Lenka (advisor) ; Janovská, Petra (referee)
Endoplasmic reticulum (ER) is a cellular organelle responsible for folding of proteins that are then transported to the various places in the cell or secreted. It is also crucial for the synthesis of triglycerides. Metabolic imbalance leads to ER stress and consequently triggers signaling pathway, which is called unfolded-protein response (UPR). The aim of this pathway is to alleviate ER stress, restore natural homeostasis and prevent death of cell. At the same time however, it activates stress kinases and other factors that may perturb insulin signaling and increase expression of proinflammatory cytokines. These signs are characteristic for human obesity, which is associated with reduced tissue's sensitivity to insulin and is considered as a disease with low level of inflammation. Recent studies have suggested that the source of proinflammatory cytokines in obesity are stressed adipocytes and macrophage infiltrated into adipose tissue. Indeed, it has been demonstrated that stress of endoplasmic reticulum is significantly increased in adipose tissue of obese individuals. Weight loss associated with reduction of adipose tissue mass decreases stress while lowers both, the production of proinflammatory cytokines and insulin resistence. This work aims to collect and discuss these new findings, which...
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Bacterial components in experimental intestinal inflammation prevention and therapy
Kverka, Miloslav ; Tlaskalová - Hogenová, Helena (advisor) ; Šedivá, Anna (referee) ; Stříž, Ilja (referee)
Although strong protective immune response is essential for preventing invasion by pathogens, equivalent responses against antigens originating from commensal bacteria can lead to chronic inflammatory diseases, such as inflammatory bowel disease (IBD). Manipulating the mucosal immune responses with microbial antigens might be an excellent tool to IBD therapy or prevention. Our aim was to gain some insight into the regulation of the intestinal inflammation and to isolate bacterial immunomodulatory components that could be used in intestinal inflammation therapy and prevention. One particular mechanism of how healthy colon tissue regulates the inflammation during acute experimental colitis is through modulation of bioavailability of glucocorticoids (GCs) in gut mucosa. Here, we show that intestinal inflammation changes the local GC metabolism, which ultimately leads to decrease in inflammatory readiness of cells in the gut mucosa and in mesenteric lymph nodes. This pre-receptor regulation of GC function could represent an important homeostatic function of the gut mucosa. The actual triggers of intestinal inflammation in IBD seem to be either microbial dysbiosis or microbes with special "pathogenic" abilities, which both could be rectified by feeding with probiotics. Here, we report that oral feeding with live...
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The cellular elements participating in the inflammatory response
CHMELOVÁ, Veronika
The issue of inflammation is a very complicated and extensive topic, which by far does not just cells that the task of this thesis. It's a proces to helping maintain the internal environment of the organism and its task is cope with external environmental influences, which may mean risk for the organism. Inflammatory cell response is the mechanism which the body defends itself against infections and corrects damaged tissue. But this process can not be understood only as a defensive reaction. Especially with persistent inflammation may evolve a disease state which leads to irreversible tissue damage. In this case, the pathologic response. The theoretical part describes the course of inflammatory reactions and focus on individual blood cells. In this part of thesis, describe their morphological structure, interaction and role in the pathophysiological process. The practical part is devoted to specifics methods which can be used to investigate inflammatory cells. I appreciate their advantages and disadvantages, and describe the principles and practices of these methods. The most basic method is the fabrication and painting the blood smear and microscopic minus differential leukocyte budget. For higher level can be considered Hematology Analyzer, in addition to white blood cells provides a range of information, including platelets and red series. The last method, which the work is concerned with flow cytometry. The huge growth in recent years, the opportunity to investigate a large number of parameters. But this places high demands on the correct interpretation of the data. The last part is concerned with mast cell and problems of its investigations both in terms of hematological and histological methods.
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Nursing care for elderly people with pneumonia.
BŘÍZOVÁ, Diana
This Bachelor Thesis on Nursing care for the elderly with lung inflammation deals with finding the specifics of treatment in elderly patients with pneumonia. It is widely known that pneumonia is one of the most serious diseases in general. This disease affects the pulmonary alveoli, respiratory bronchioles and pulmonary intestinum. Most lung inflammations are of an infectious origin. The most vulnerable group of people affected by this disease are not only smokers, diabetics, people with impaired immunity but mostly seniors. Today, despite the latest treatment methods, pneumonia is a frequent and high-risk disease. Worldwide, pneumonia takes the third place in causes of death, and the first place in infectious diseases. For this reason, it is important to know all the precautions and the quality nursing care needs the nurse to know all information on this disease (Vyšínová 2012; Musil 2007). The Bachelor Thesis consists of theoretical and practical parts. The theoretical part deals with geriatrics, specific changes in old age, pulmonology, pneumonia classification, clinical picture, diagnosis, treatment, complications, prevention, and especially nursing. In this section we deal with inhaled therapy, pharmacology, adequate hydration, catering, measuring physiological functions, monitoring pain, relaxation, emptying, respiratory care, hygienic care and communications. The practical part consists of qualitative research method. In this research, we have achieved goals through semi-structured interviews with nurses and seniors. The interviews conducted in a standard pulmonary and internal hospital departments in České Budejovice, are included in our Thesis. In this Thesis we have set four objectives. The first objective: to find out the specifics of nursing care for seniors with pneumonia. The second objective: to determine how nurses perceive nursing care for the elderly with pneumonia. The third objective: to find out how the elderly perceived nursing care. The fourth objective: to determine the level of education of senior citizens before they leave hospital for the home environment. Each objective was determined by a research question. Research question 1: What are the specifics of nursing care for the elderly with pneumonia? Research question 2: What is the perception of nursing care from the perspective of nurses? Research question 3: What is the perception of nursing care from the perspective of seniors? Research question 4: How do we assess education of seniors before they leave hospital for the home environment? The outcome of this work is a methodical procedure of nursing (see Annex 4). This Thesis can be used by students of nursing as well as study materials
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The Scintigrafic detection of inflammation in patients examined in the Motol University Hospital since 2007 to 2009.
BRHEL, Jan
This bachelor?s thesis outlines an attempt to describe, to the professional public, the principles of imaging of inflammation in the human body using the methods of nuclear medicine. Two types of imaging of inflammation are described: scintigraphy of labeled leukocytes in vitro and scintigraphy of labeled leukocytes in vivo. The course of the examination, preparation and use of radiopharmaceuticals, indications, contraindications, imaging, etc. are described for both types of methods. The next section of thesis consists in comparsion and evaluation of the data obtained from the hospital system (UNIS), relating to the above tests in 2007 ? 2009 at the University Hospital in Motol employing European standards in nuclear medicine. The results are presented in the from of tables and graphs. The work was intended to confirm the hypothesis mentioned in the bachelor?s thesis and to create an overall idea of the quantities of these tests. Last but not least, it is useful to compare the competitiveness of nuclear medicine with other imaging methods, such as computer tomography and magnetic resonance imaging.
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