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Determination of hyaluronan by electromigration methods
Bártová, Adéla ; Mravcová, Ludmila (referee) ; Řezáčová, Veronika (advisor)
This thesis is focused on description hyaluronic acid, characterization of its behaviour in water solutions, its chemical-physical properties and its using in different industrial sectors. The next part deals with possibilities of its analysis. The following section of the thesis is focused on electromigration techniques with special attention dedicated to capillary electrophoretic methods, especially capillary zone electrophoresis. The aim of this thesis is optimization of the method of determination of hyaluronic acid in water solutions by capillary zone electrophoresis.
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Evaluation of basic characteristics of thermal water with separation methods
Skálová, Lucie ; Mravcová, Ludmila (referee) ; Vávrová, Milada (advisor)
The presence of inorganic ions is one of the criteria for thermal water assessing. The most important anions that affect the water quality and allow their use for therapeutic purposes, include sulfates, chlorides, nitrates and bicarbonates. Ions (as sodium, potassium, calcium and magnesium) are the significant cations. The ion chromatography is used for separation of these substances and also some methods based on electrophoretic migration of ions in an electric field. The electromigration method of isotachophoresis was chosen for determination of selected ions in water samples collected from thermal boreholes in Pasohlávky.
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The electromigration methods and their use in the analysis of drugs.
Tučková, Dominika ; Tulková, Tereza (referee) ; Řezáčová, Veronika (advisor)
This bachelor thesis deals with the determination of septonex, effective component in medical agents, using capillary isotachophoresis. The main aim is optimization of this method for fast, sensitive and inexpensive determination of septonex. In bachelor thesis chemical and physical properties of septonex are introduced as well as its negative and positive properties, which can be used in medicine, and summary of possible methods of analysis. Further this thesis gathers information about drugs containing septonex and available on Czech market. Following chapters of the thesis summarize theory of electromigration methods, their principles, analytical devices and their use for septonex analysis. Finally, the thesis presents optimal conditions for analysis and results of determination of septonex in drug Septonex spray, which is available on Czech market, using capillary isotachophoresis. Content of determined septonex is compared to amount of septonex contained in this drug, which is declared by producer, in this thesis.
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Stanovení vybraných iontových herbicidů v povrchových vodách
Ondračka, Tomáš
The thesis deals with the determination of selected ion herbicides in surface waters and in the first part describes the herbicides. Given the broad spectrum herbicide further work deals mainly with glyphosate. The next section describes the electromigration methods and the methods by which the herbicides were determined. Finally, it describes how the development of an electrolyte system along with the use of graphical possibilities of computer technology. In conclusion are presented and commented on the results of measurements of glyphosate in surface waters, compared with foreign literature and assessing the suitability of methods for the analysis of contaminants in the environment.
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Využití elektromigračních metod ke stanovení těžkých kovů v odpadních vodách
Ondračka, Tomáš
The diploma thesis deals with the determination of selected heavy metals in wastewater, while the first part describes electromigration methods and individual heavy metals. In the next part of the thesis is describe, the problems of sewage sludge and methods commonly used for determination of heavy metals in wastewater. The practical part of the thesis describes the development of the electrolyte system together with the use of computer technology. Following is a discussion of the possibility of removing heavy metals from wastewater. In conclusion, the results of heavy metal measurements, comparison with foreign literature and assessment of suitability of the method for analysis of hazardous substances at low concentrations in the environment are presented and commented.
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Determination of hyaluronan by electromigration methods
Bártová, Adéla ; Mravcová, Ludmila (referee) ; Řezáčová, Veronika (advisor)
This thesis is focused on description hyaluronic acid, characterization of its behaviour in water solutions, its chemical-physical properties and its using in different industrial sectors. The next part deals with possibilities of its analysis. The following section of the thesis is focused on electromigration techniques with special attention dedicated to capillary electrophoretic methods, especially capillary zone electrophoresis. The aim of this thesis is optimization of the method of determination of hyaluronic acid in water solutions by capillary zone electrophoresis.
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The electromigration methods and their use in the analysis of drugs.
Tučková, Dominika ; Tulková, Tereza (referee) ; Řezáčová, Veronika (advisor)
This bachelor thesis deals with the determination of septonex, effective component in medical agents, using capillary isotachophoresis. The main aim is optimization of this method for fast, sensitive and inexpensive determination of septonex. In bachelor thesis chemical and physical properties of septonex are introduced as well as its negative and positive properties, which can be used in medicine, and summary of possible methods of analysis. Further this thesis gathers information about drugs containing septonex and available on Czech market. Following chapters of the thesis summarize theory of electromigration methods, their principles, analytical devices and their use for septonex analysis. Finally, the thesis presents optimal conditions for analysis and results of determination of septonex in drug Septonex spray, which is available on Czech market, using capillary isotachophoresis. Content of determined septonex is compared to amount of septonex contained in this drug, which is declared by producer, in this thesis.
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Evaluation of basic characteristics of thermal water with separation methods
Skálová, Lucie ; Mravcová, Ludmila (referee) ; Vávrová, Milada (advisor)
The presence of inorganic ions is one of the criteria for thermal water assessing. The most important anions that affect the water quality and allow their use for therapeutic purposes, include sulfates, chlorides, nitrates and bicarbonates. Ions (as sodium, potassium, calcium and magnesium) are the significant cations. The ion chromatography is used for separation of these substances and also some methods based on electrophoretic migration of ions in an electric field. The electromigration method of isotachophoresis was chosen for determination of selected ions in water samples collected from thermal boreholes in Pasohlávky.
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Stanovení vybraných kovů pomocí kapilárních elektroforetických technik
Kleclová, Jaroslava
There is described a electromigration separation method for the analysis of low concentrations of copper with the possibility to detect a Wilson disease in my thesis. There were developed a methodology and propitious electrolyte systems, which can pre-concentrate copper prior to its on-line analysis. The developed electrolyte system for analysis had the following composition: LE1 (1.10-2 M CH3COOH, 2.10-2 M CH3COONH4, 2.10-4 M SPADNS), LE2 (1.10-2 M CH3COONH4, 1.10-2 M NH4OH, 1.10-4 M BKP, 5.10-5 M PAR, 5.10-3 M C6H17N3O7) and DE (7.10-5 M Cu(CH3COO)2, 1.10-3 M CH3COONH4 a 1.10-2 M CH3COOH). With a suggested electrolyte was achieved almost seventyfold accumulation of copper during 42 minutes, which lowered the concentration detection limit on the level convenient for the detection of Wilson disease. Complete analysis took about 98 minutes. There was accomplished a conductometric detection of copper in my thesis, however the developed electrolyte system can also analyze a copper by photometric detection, which is more sensitive.
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