National Repository of Grey Literature 4 records found  Search took 0.00 seconds. 
Fyziologické změny kmenů Saccharomyces cerevisiae s delecemi v genech pro PDR transportéry
Hlaváček, Otakar ; Váchová, Libuše ; Palková, Z.
We focused on comparison of the development and behaviour of S. cerevisiae strains overproducing various PDR transporters to those that have genes for some of them deleted. Besides a spectrum of physiological parameters, we analysed also expression changes of selected genes and measured reduced cytochrome spectra of these strains to find out the state of their respiration
Interakce xenobiotik s MDR pumpami u S. cerevisiae
Hendrych, T. ; Zlámalová, Marta ; Gášková, Dana ; Pichová, Alena ; Sigler, Karel
The interactions of a number of xenobiotics with two main S. cerevisiae MDR pumps, Pdr5p and Snq2p were studied. The results showed that some compounds do not interact with MDR pumps whereas others affect one or another of the pumps, causing a loss of their ability to transport an indicator substrate out of the cells. This apparent inactivation of the pumps can be due to competition for transport with the indicator substrate or to actual (noncompetitive) inhibition of the pumps
Tvorba proteinů Ato v tekutých kulturách koreluje s uvolňováním amoniaku
Strachotová, Dita ; Palková, Z. ; Váchová, Libuše
pH of the media changes during growth of both shaken or static liquid S. cerevisiae similarly to pH of the agar under colonies. Likewise in colonies, production of all Ato proteins in liquid cultures initiates during medium alkalization
Amoniaková signalizace u kolonií Saccharomyces cerevisae: Exprese transportérů aminokyselin u kmenů defektních v SPS sensoru
Harant, Karel ; Váchová, Libuše ; Palková, Z.
The capability of yeast colonies to produce ammonia depends on correct function of SPS sensor composed of Ssy1p, Ssy5p and Ptr3p. The obtained data indicate that SPS sensor influences the expression of amino acid transporter genes in yeast colonies by different way when compared with its function in yeast liquid cultures

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