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National Repository of Grey Literature 4 records found  Search took 0.00 seconds. 
Cyclické peptidy a depsipeptidy jako markery houbových infekcí
 Jegorov, A. ; Sulc, M. ; Ulrych, A. ; Navrátil, P. ; Curn, V. ; Kratochvíl, B. ; Havlíček, Vladimír
Fungi produce cyclic peptides and depsipeptides by multifunctional synthetases.
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Konstrukce neoznačených mutantů v genech kódujících Ser/Thr proteinkinasy a fosfoproteinfosfatasu Pseudomonas aeruginosa a analýza jejich vlastností
 Nedvědová, Jana ; Lněnička, Petr ; Hercík, Kamil ; Branny, Pavel
To investigate the importance of individual protein kinases and phosphatases in P. aeruginosa physiology we prepared the single and double mutants in the coresponding genes. Single mutants in protein kinase genes stk1, ppkB and protein phosphatase gene stp1 were prepared by the method of gene replacement with subsequent excision of antibiotic cassettes using Flp-FRT recombination system. Two-dimensional gel electrophoresis was used to analyze the patterns of phosphoproteins obtained in in vitro protein kinase assays with the cell-free lysates of wild type as well as all mutant strains.
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Vztah mezi modulární strukturou serin/threonin proteinkinasy eukaryontního typu StkP z Streptococcus pneumoniae a její funkcí
 Pallová, Petra ; Přenosilová, Lenka ; Nováková, Linda ; Branny, Pavel
In order to determine a cellular localization of protein kinase StkP we prepared its epitope-labelled form expressed in S. pneumoniae. The full-length form of StkP was detected with monoclonal antibody in the membrane fraction. In vitro autophosphorylation assays confirmed this finding and phosphorylated product of corresponding mass was detected in the membranes. These results clearly showed that StkP is a membrane-associated protein.
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Identifikace genů a proteinů, regulovaných Ser/Thr proteinkinasou Streptococcus pneumoniae, mikročipovou metodou a analýzou 2-D gelů
 Přenosilová, Lenka ; Nováková, Linda ; Branny, Pavel
To identify the genes whose expression is controlled by protein kinase StkP we examined the transcription profile of DstkP mutant by DNA microarray technology. The transcript analysis revealed the StkP-dependent expression of many potential target loci. Out of 2,300 genes evaluated, 49 were found to be specifically and strongly upregulated in the DstkP strain, whereas 48 were downregulated. The data obtained from transcription profile were further extended by proteomic studies and mass spectrometry.
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