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A MODIFIED MATHEMATICAL MODEL OF HUMAN VENTRICULAR CARDIOMYOCYTE INCORPORATING SEPARATE T-TUBULAR AND SURFACE DYADS AND SUBMEMBRANE SPACES
Pásek, Michal
;
Bébarová, M.
;
Christé, G.
Intracellular Ca2+ load and Ca2+ transient are considerably dependent on distribution of sarcolemmal Ca2+ pump and Na+-Ca2+ exchanger between the t-tubular and surface membranes in the presence of separate dyadic and subsarcolemmal spaces in rat ventricular cell model. To explore analogical phenomenon in human, we modified our previously published model of human ventricular myocyte. When the t-tubular fractions of Na+-Ca2+ exchanger and of sarcolemmal Ca2+ pump were increased to the newly proposed value of 0.95 in the modified model, the following changes were observed at 1 Hz steady-state stimulation: a shortening of the action potential duration at 90% repolarisation by 6% and an increase of the cytosolic Ca2+ transient by 22%. Further analysis revealed a critical role of Ca2+ concentration changes in the subsarcolemmal spaces and consequent change in cellular Ca2+ cycling in this effect.
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Experimental modelling of phonation using artificial models of human vocal folds and vocal tracts
Horáček, Jaromír
;
Radolf, Vojtěch
;
Bula, Vítězslav
;
Košina, Jan
The study provides information on experimental research on a complete 1:1 scaled model of human phonation. The model includes human lungs, the trachea, the laryngeal part with artificial vocal folds and the vocal tracts designed for different vowels. The measurement set up enables modelling the time signals not easily measured in humans during phonation as for example fluctuations of the subglottic, laryngeal and oral pressures measured simultaneously with the glottis opening and the glottis area registered by a high-speed camera. The simulation of phonation is performed in the ranges of the airflow rate and the subglottic pressure typical for a normal humans' physiology.
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