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Objemová vizualizace velkých biologických tkáňových vzorků
Čapek, Martin ; Kubínová, Lucie ; Janáček, Jiří ; Hána, K. ; Smrčka, P.
We apply volume reconstruction for visualization of a biological specimen greater than the field of view of a confocal laser scanning microscope. Prior to the volume reconstruction, large specimens are cut into thin physical slices. The first step of volume reconstruction is acquisition of digital volume images (spatial tiles which overlap) from all studied physical slices. The second step is horizontal merging of overlapping spatial tiles of the same physical slice using a registration algorithm based on a mutual information and translation. The third reconstruction step is vertical merging of digital volumes of successive physical slices using an elastic registration algorithm based on B-splines. The resulting large digital volumes are visualized by a VolumePro hardware board that provides volume rendering in real-time. In this paper we show a reconstruction of a chick embryonic kidney.
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Dvoukanálový, komplexní, bioimpedanční signál pro vyhodnocení hemodynamiky
Halámek, Josef ; Vondra, Vlastimil ; Jurák, Pavel ; Verner, Petr
The complex bioimpedance signals measured in the aortal and pulmonary artery directions simultaneously are discussed with respect to the information about the heart activity. All four signals carry some information about the blood flow, this information is partly equivalent (only SNR improvement and some artefact minimizations may be achieved by processing all signals) and partly non-equivalent (different behaviour according to excitation of the cardiovascular system). The more detailed analysis of time evolution of signal parameters according to the used excitation may contribute to new information about the hemodynamic system.
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