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Different metabolic pathwazy of the origin of hyperhomosysteinemia
Přistoupilová, K. ; Přistoupil, T. I. ; Navrátil, Tomáš ; Heyrovský, Michael ; Šenholdová, Z. ; Kohlíková, E. ; Petr, M.
Our voltammetric method of estimation of thiodiglycolic acid (TDGA) in urine was used in case of a woman suffering from rare inborn inability to metylate homocysteine (HoCySH). The aim of this pilot study was to shed more light on the interrelationship among vitamin B.sub.12./sub., folates, and betaine used for her cure. On the basis of the achieved results we were able to modify our earlier published metabolic scheme. It comprises 3 metabolic pathways starting from serine, and three metabolic pathways starting from HoCySH.
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Změny koncentrace TDGA v moči jako možný marker metabolismu thiosloučenin
Přistoupilová, K. ; Přistoupil, T. I. ; Šenholdová, Z. ; Navrátil, Tomáš ; Heyrovský, Michael ; Fenclová, Z.
The new simple voltammetric method we described recently, proved already to be useful in determining thiodiglycolic acid (TDGA) levels in urine samples of people influenced by some chemicals, victuals and pharmaceuticals. The present pilot study is a continuation of our previous work devoted to test general feasibility of the method. We concentrated upon time dependent quantitative changes of TDGA excretion into urine of selected volunteers. The TDGA level in all morning samples was the highest in comparison with samples collected during the day. The TDGA level was dependent on the health condition of each person involved. However, a further marked increase of TDGA in the morning urine was observed individually, in general when vitamin B12 had been administered in the evening the day before. Vitamin B12 is known to play an important role in the metabolism of one- and two-carbon units, of homocysteine and of sulfhydryls in general. Certain differences in excretion course of TDGA.
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