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Application of pressurized hot water for etching of microfluidics
Vašina, M. ; Grym, Jakub ; Foret, František
The subject of this study is a new method of glass etching and its application in microfluidics. Commonly, hydrofluoric acid is used for glass etching. In this study we have tested a new method of glass etching using pressurized hot water. Test motives were prepared using photolitography and metal sputtering on the glass substrate and the etching was performed in a newly developed device capable to compress and heat water to its critical point. Electron microscopy and profilometry have been used for analyzing the surface of the etched glass substrate.
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High pressure modification of the simple automated liquid chromatographic system for splitless nano column gradient separations
Šesták, Jozef ; Kahle, Vladislav
Our simple liquid chromatographic system for splitless gradient nanocolumn separations at backpressure up to 50 bars was modified to run analysis under the pressure up to 300 bars. Programmable syringe pump was equipped with a 250-L high-pressure syringe and mobile phase gradients were created and stored in relatively long capillary. Available linear gradients of volumes from 5 to 50 L traced by uracil are presented. Sample introduction was performed as separate step and real large volume of sample (tens of microliters) could be injected and preconcentrated on-column. Relative standard deviation of retention times and peak areas were received (RSD < 0.3 % and RSD < 5 % respectively).
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Low-cost 3D-printed fluorescence detector for capillary electrophoresis
Přikryl, Jan ; Foret, František
We have verified the possibility of Fused Deposition Modeling (type of additive manufacturing) for lab-made construction of inexpensive fluorescence detector for capillary electrophoresis using commercially available optical components. Sensitivity of fabricated and assembled detection system was tested by fluorescein solutions. Limits of detection were determined for several tested arrangements.
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Thin metal films for detection and preconcentration
Podešva, Pavel ; Foret, František
We have tested CE separation of selected samples in capillaries with polydopamine modified surface. The capillaries were modified by polydopamine or polydopamine with an additive. The polydopamine coating with additive represents a simple and effective procedure for capillary alteration by another modificator such as hydroxyethyl cellulose, hydroxypropyl cellulose etc. In this work, we represent separation data from PrinCE system with UV detection for peptides, proteins and oligosaccharides labeled by 2-aminobenzoic acid.
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Polydopamine coated capillaries for CE separations
Partyka, Jan ; Foret, František ; Vu, T. T. ; Sudor, J.
We have tested CE separation of selected samples in capillaries with polydopamine modified surface. The capillaries were modified by polydopamine or polydopamine with an additive. The polydopamine coating with additive represents a simple and effective procedure for capillary alteration by another modificator such as hydroxyethyl cellulose, hydroxypropyl cellulose etc. In this work, we represent separation data from PrinCE system with UV detection for peptides, proteins and oligosaccharides labeled by 2-aminobenzoic acid.
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Electrophoretic mobility measurements of a multivalent red dye SPADNS
Pantůčková, Pavla ; Kubáň, Pavel ; Boček, Petr
Measurements of the electrophoretic mobility of an anionic dye 4,5-dihydroxy-3-(p-sulfophenylazo)-2,7- naphthalene disulfonic acid trisodium salt (SPADNS), which is a commonly used anionic dye, are described in this contribution. Electrophoretic mobilities of SPADNS were measured in various buffer solutions covering the pH range of 2.4–9.1. Effective electrophoretic mobilities of SPADNS measured in these buffers varied between 50.5 x 10–9 m2/Vs and 60.5 x 10–9 m2/Vs with an average effective mobility of 55.0 x 10–9 m2/Vs.
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IEF and HPLC-based methods for efficient bacterial characterization
Kubesová, Anna ; Šalplachta, Jiří ; Moravcová, Dana ; Horká, Marie
Pectobacterium and Dickeya species are responsible for blackleg and tuber soft rot diseases on crop and ornamental plants. Recently, a more virulent and aggressive Dickeya type of E. chrysanthemi was described and it becomes serious problem for potato production in Europe. In this study, we suggest procedures using two commonly available laboratory techniques, gel isoelectric focusing and liquid chromatography, for differentiation and characterization of Pectobacterium and Dickeya species. We have confirmed that fingerprinting approach can be used for bacterial differentiation, which can be further used in rapid diagnosis of plant disease.
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