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Methylace histonu H3K9 v lidských krevních buňkách a v granulocytech pacientů s myeloidní leukemií
Lukášová, Emilie ; Falk, Martin ; Kořistek, Z. ; Kozubek, Stanislav ; Grigoryev, S. ; Kozubek, Michal ; Ondřej, Vladan ; Kroupová, I.
Common heterochromatin antigenic protein markers while present in human blood progenitor CD34+ cells, differentiated lymphocytes and monocytes are absent in neutrophile granulocytes and, to large extent, in eosinophiles. In acute CML and AML, strong methylation of H3K9 and all isoformes of HP1 are detected. In chronic forms of CML, no strong correlations among the level of histone methylation, disease progression and modality of treatment were observed. Reprogramming of leukemia HL60 cells to terminal differentiation by retinoic acid does not eliminate histone H3K9 methylation and the presence of HP1 isoformes from differentiated granulocytes. Our study shows for the firs time that histone H3 methylation may be dramatically changed during normal cell differentiation.
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Využití opticky zachycené sondy pro studium velmi slabých interakcí na molekulární úrovni - přehled
Zemánek, Pavel ; Jonáš, Alexandr
A micron-sized dielectric particle confined in a laser trap can be employed as a probe for the measurement of forces in the range from piconewtons to hundreds of piconewtons. Thus, if a biological system of interest (e.g. DNA molecule, single myosin or kinesin molecule, cell membrane) is attached to such a probe, its mechanical and dynamical properties (elasticity, viscosity, forces associated with movement) can be studied during its interaction with the environment with unprecedented resolution. This article introduces the basic principles of the laser trapping and force measurement and illustrates on several examples the great potential of the light-based force transducer for exploiting non-invasively the dynamics of molecular systems
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