Národní úložiště šedé literatury Nalezeno 3 záznamů.  Hledání trvalo 0.00 vteřin. 
Determination of Variation Components from Geodetic Micronetwork Adjustment
Váchová, Hana
This paper deals with the design of a procedure to determine the accuracy of a total station. It is based on the surveying of a geodetic micronetwork and subsequent evaluation of the measured data using the created software tool. The calculation works on the principle of least squares adjustment with the application of the Förstner method of estimation of variation components. In addition, the calculation is enriched by the possibility of determining residual errors of the prism constants.
Functional analysis of novel F\dindex{1}-ATPase subunit in \kur{Trypanosoma brucei}
VÁCHOVÁ, Hana
Although F1-ATPase is extremely conserved among organisms, a putative subunit p18 was identified in Trypanosoma brucei F1-ATPase complex. To explore its function in the procylic, bloodstream and dyskinetoplastic trypanosomes, three different RNAi cell lines were created. Upon p18 silencing the F1-moiety structural integrity was impaired suggesting that p18 is indeed a bona fide subunit of this complex. Since F1-ATPase is crucial for the bloodstream form survival, its potential inhibitor from the 4-oxopiperidine-3,5-dicarboxylates class (JK-11) was examined. JK-11 inhibited growth of the bloodstream trypanosomes, decreased mitochondrial membrane potential and reduced ATPase and ATP synthase activity in mitochondrial lysates. Our results suggest that JK-11 may act on FoF1-ATP synthase/ATPase and its inhibition may contribute to the cytotoxicity of this drug.
Charakterizace inhibičních vlastností proteinu TbIF1 vůči mitochondriálnímu komplexu F1-ATPázy u bičíkovce \kur{Trypanosoma brucei}.
VÁCHOVÁ, Hana
To map and define the minimal inhibiting sequence of the inhibitory peptide TbIF1 and to improve its inhibiting property, a series of the N- and C- terminal truncations of TbIF1 was created. The mutated recombinant TbIF1 his-tagged proteins were over-expressed and purified from the E.coli cells and their binding and inhibiting properties were tested using purified F1-ATPase complex.

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3 Váchová, Hana
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