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The effect of salts on the hydrolytic stability of biopolymer carriers with antibacterial substances
Tertinská, Martina ; Brtníková, Jana (oponent) ; Vojtová, Lucy (vedoucí práce)
This diploma thesis deals with observing the effects of salt on the stability of biopolymer carriers made of protein and polysaccharide. The theoretical part is concerned with biomaterials and materials for sample preparation and also deals with the current situation of treatment of infected wounds with various materials. Within the experimental part, the carriers were prepared in two different buffers with different concentrations of sodium chloride in order to stabilize bioactive proteins, peptides or enzymes generally used for carrier entrapment. In every series of samples there were prepared both crosslinked and non-crosslinked samples. Properties like hydrolytic stability and swelling ratio were tested and the effect of salt concentration on sample stability was observed. Since the optimal salt concentration has been established, antibacterial enzyme was entrapped into all carriers and its release was monitored. Whereas at non-crosslinked samples significant differences between salt and salt-free samples both stability and enzyme release have been monitored, at crosslinked samples the differences were neglectable. However, in all cases the enzyme released from foamed samples according to first order kinetics, which is concentration dependent. Based on the results from disk diffusion test provided on different strains of S. aureus, the concentration of 325 mg/ml, have been chosen for my further work. Prepared carriers with antibacterial enzyme could be used as both- fast or prolonged releasing wound dressings for Staphylococcus aureus or methicillin-resistant Staphylococcus aureus (MRSA) infected tissue.
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