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Isosporan oocysts in the faeces of bank voles (Myodes glareolus; Arvicolinae, Rodentia): real parasites, or pseudoparasites?
TREFANCOVÁ, Aneta
This study concerns clarification of the origin of infections of arvicoline rodents with Isospora spp. based on three different approaches: phylogenetic analyses of three genes (18S rRNA, COI and COIII), morphological and morphometrical analyses, and experimental infection. Field collections, parasitological examinations of samples, microscopy, DNA extraction, PCR, and computational analyses were employed during the course of this study.
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Morfologické znaky a populačně-genetická struktura kokcidií parazitujících u hrabošovitých hlodavců; objasnění původu isosporových infekcí u hrabošovitých hlodavců
TREFANCOVÁ, Aneta
The first part of the thesis concerns phylogenetic relationships of eimerian coccidia from arvicoline rodents based on nuclear 18S rRNA, and mitochondrial COI and COIII genes, as well as morphological and morphometrical analyses of the eimerian oocysts. Population-genetic structure of eimerian coccidia was also analyzed and haplotype networks were constructed. The second part of the thesis is based on elucidation of the origin of infections of arvicoline rodents with Isospora spp. using three different approaches - phylogenetic analyses (18S rRNA, COI, and COIII genes), morphological and morphometrical data, and experimental infections. Techniques of field parasitology, laboratory methods of parasitological examination, molecular-genetic diagnostics, phylogeny, and taxonomy were used in the course of both parts of the study.
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