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Národní úložiště šedé literatury Nalezeno 2 záznamů.  Hledání trvalo 0.01 vteřin. 
Molecular and biochemical characterization of serine protease SmSP1 in \kur{Schistosoma mansoni}
 OPAVSKÝ, David
SmSP1 is a chimerical serine protease consisted of three domains (cub, LDLa and trypsin-like) and found in Schistosoma mansoni. Its characterization was performed by molecular techniques such as PCR screen, qRT-PCR and RNA interference (RNAi) to gain information about expression profile, level expression and susceptibility to RNAi. Further, protein expression was carried out to gain an antigen for immunization and recombinant for biochemical studies. Results of PCR screen and qRT-PCR suggested possible function of SmSP1 in egg and adult stages but SmSP1 gene was not found susceptible to RNAi in NTS. Recombinant from E. coli was successfully used for immunization. Active recombinant was likely expressed in Pichia pastoris but expression conditions are unstable and expression optimization is necessary.
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Studium genu \kur{disc overgrown} v imaginálních discích \kur{Drosophila melanogaster}
 OPAVSKÝ, David
The aim of this bachelor{\crq}s thesis was to make a Drosophila line with a gene set that induces a creation of mitotic clones in wing discs and eye discs. Seven different Drosophila stocks from various sources were used in genetic crossing to obtain such line. The mitotic-clone area was composed of either cells that were homozygous for GFP or cells that were homozygous for dco3 mutation. The dco3 is a recessive lethal mutation leading to death in pupae. By induction of a small number of cells homozygous for dco3 it was possible to observed the changes in adult tissues with mutant clones.
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