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Atelocollagen processing, gelation and characterization
 Zubal, Lukáš ; Podzimek,, Štěpán (oponent) ; Migliaresi, Claudio (oponent) ; Vojtová, Lucy (vedoucí práce)
Proposed thesis deals with the characterization, processing and gelling of soluble collagen. A literature review summarizes position of collagen at the transplant market, explains its application and describes different types of collagen. Various methods of characterization of soluble collagen are analysed in detail and different ways of its gelation are thoroughly discussed. The experimental part is divided into two sections. The first section of experimental part deals with the dissolution and gelation of soluble collagen in pressurized CO2 atmosphere. Compared to the other similar methods, our method operates in a relatively low pressure (up to 0.9MPa) and and low temperatures (close to 0°C) that does not denature soluble collagen (works at low temperatures). This method can be used for fast formation of transparent gels containing very thin fibers. Speed of gelation and transparency of gels give a new potential to the concept of ophthalmology and should bring significant benefits to injectable products, creating microspheres and 3D printing. The second section describes the unique characterization of soluble collagen in native state by Asymmetric Flow Field Flow Fractionation - Multi Angle Light Scattering (AF4-MALS) method. The obtained molar mass perfectly fits the expected molar mass. Native and denatured collagen can readily be identified on the conformational graph. This new method provides stable and consistent results in comparison with other established methods. AF4-MALS method can be used either to optimize yields and purification during the production of soluble collagen or for sensitive detection of collagen denaturation during processing.
Úplný záznam
Atelocollagen processing, gelation and characterization
 Zubal, Lukáš ; Podzimek,, Štěpán (oponent) ; Migliaresi, Claudio (oponent) ; Vojtová, Lucy (vedoucí práce)
Proposed thesis deals with the characterization, processing and gelling of soluble collagen. A literature review summarizes position of collagen at the transplant market, explains its application and describes different types of collagen. Various methods of characterization of soluble collagen are analysed in detail and different ways of its gelation are thoroughly discussed. The experimental part is divided into two sections. The first section of experimental part deals with the dissolution and gelation of soluble collagen in pressurized CO2 atmosphere. Compared to the other similar methods, our method operates in a relatively low pressure (up to 0.9MPa) and and low temperatures (close to 0°C) that does not denature soluble collagen (works at low temperatures). This method can be used for fast formation of transparent gels containing very thin fibers. Speed of gelation and transparency of gels give a new potential to the concept of ophthalmology and should bring significant benefits to injectable products, creating microspheres and 3D printing. The second section describes the unique characterization of soluble collagen in native state by Asymmetric Flow Field Flow Fractionation - Multi Angle Light Scattering (AF4-MALS) method. The obtained molar mass perfectly fits the expected molar mass. Native and denatured collagen can readily be identified on the conformational graph. This new method provides stable and consistent results in comparison with other established methods. AF4-MALS method can be used either to optimize yields and purification during the production of soluble collagen or for sensitive detection of collagen denaturation during processing.
Úplný záznam

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