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Quantum dot-based immunoprobe for optical and electrochemical detection
Dvořáková, V. ; Čadková, M. ; Datinská, Vladimíra ; Chałupniak, A. ; Korecká, L. ; Merkoci, A. ; Klepárník, Karel ; Foret, František ; Bílková, Z.
Three-stage labeling strategy allows the preparation of specific conjugates composed of required antibodies and quantum dots as an extremely sensitive bio-probe applicable in varied immunoassays with following optical and electrochemical detection. This approach is essentially based on carbodiimide chemistry however each single step of whole procedure is separated and controlled which makes this applied protocol oriented, highly efficient and it enables gain of pure antibody-conjugated quantum dots in ready-to-use condition.

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