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National Repository of Grey Literature

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Rpg1p/eIF3a se spojuje s mitochondriemi v Saccharomyces cerevisiae Hašek, Jiří ; Janatová, Ivana ; Špryngar, Martin ; Wolinski, H. ; Kohlwein, S. D. RPG1/TIF32, the essential gene of Saccharomyces cerevisiae, encodes eIF3a subunit of the translation initiation factor 3. We show here that both temperature sensitive mutant of Rpg1 strongly differ in their distribution at permissive temperature, distribution of the Rpg1-1-GFP fusion is cytosolic, the fusion protein Rpg1-2-GFP was accumulated in large cytosolic aggregates. Using the life image analysis with confocal laser scanning microscope we found that these granules could be inherited to daughter cells and obviously associate with mitochondria. Detailed record

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