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National Repository of Grey Literature

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Growth screening of Rhodospirillum rubrum using microcalorimetry Vetchý, Antonín ; Slaninová, Eva (referee) ; Krouská, Jitka (advisor) The presented work deals with the study of the growth of the bacterium Rhodospirillum rubrum using microcalorimetry. The theoretical part is devoted to the characterization of the investigated microorganism and the description of the main measurement method. Two strains of the mentioned bacterium were selected for the measurement – the wild type strain and the mutant strain with a deletion of the gene for PHA synthase. Firstly, the optimization of measurement conditions was performed, including the examination of various values of the initial optical density. The main objective of the work was to compare the growth of both strains of R. rubrum on different substrates differing in carbon source. Normalized heat flow curves obtained by microcalorimetry, and growth curves measured by the traditional spectrophotometric method were used for comparison. In conclusion, replicable measurement conditions were established, providing comparable results for both strains of bacteria. The most suitable medium for biomass growth of both strains of the bacterium Rhodospirillum rubrum appears to be the SYN FAY medium, while the least suitable medium seems to be the SYN AY medium containing acetate, where partial inhibitory effects on bacterial growth were observed. The results of the analysis using the microcalorimeter were further confirmed and extended by measuring growth curves on an ELISA reader. Detailed record

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