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Fabrication and characterization of advanced microfluidic chips
Vejrosta, Jakub ; Novotný,, Jakub (referee) ; Plichta, Tomáš (advisor)
This diploma thesis deals with the design, fabrication and verification of microfluidic chips that can be used to sort particles using surface acoustic and standing surface acoustic waves. The sorting function of this chip is based on the piezoelectric properties of the substrate. For this reason, lithium niobate was selected as the substrate. The acoustic waves are generated by interdigital transducers placed directly on the surface of the substrate, which were fabricated by metal layer evaporation, optical lithography and reactive ion beam etching techniques. The microfluidic channels were made from a biocompatible polymer, polydimethylsiloxane, and subsequently bonded to the substrate with the interdigital transducers already prepared. The correctness of fabrication and assembly of the optimized microfluidic chips was subsequently verified experimentally by sorting polystyrene particles of different diameters. Another application of these chips was the preparation of oriented structures of the bacterium Cupriavidus necator H16 using surface acoustic waves.
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Characterization of microfludic chips
Vejrosta, Jakub ; Enev, Vojtěch (referee) ; Venerová, Tereza (advisor)
Nowadays, when we are moving to smaller and smaller scales, microfluidics is being used, which helps us to move from fluid devices to the dimensions of microfluidics, which may have a great application in the future. This work deals with the correct preparation and production of a microfluidic chip. Which consist of two parts. One part is the polydimethylsiloxane (PDMS) part of the chip, and the other is the coverslip. In this work, the production process of this chip is improved. The functionality of these chips and the time dependence of the age of the chip and the polydimethylsiloxane from which the chips are made were tested using fluorescence microscopy.
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Surface acoustic wave microfluidic chip for rapid cell sorting
Vejrosta, Jakub ; Maňka, Tadeáš ; Plichta, Tomáš ; Šerý, Mojmír
This work revolves around microfluidics and micro-objects manipulation in general. In our device, we are producing surface acoustic wave (SAW). The SAW can be used to sort particles by their size or physical properties and separate them. In medical analysis SAW on microfluidic chip can be used to pre-sorting of body fluids or blood serum for further optical or chemical analysis. The wave is produced by pair of interdigital transducers (IDTs), which are situated on opposite sides of microfluidic channel. As our active material we have used lithium niobate crystalline material for its great piezoelectric properties. This work is also the precursor for measuring the rheological properties of a medium, such as micro-viscosity, via the measurement of the trajectory of a flow trace.
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Characterization of microfludic chips
Vejrosta, Jakub ; Enev, Vojtěch (referee) ; Venerová, Tereza (advisor)
Nowadays, when we are moving to smaller and smaller scales, microfluidics is being used, which helps us to move from fluid devices to the dimensions of microfluidics, which may have a great application in the future. This work deals with the correct preparation and production of a microfluidic chip. Which consist of two parts. One part is the polydimethylsiloxane (PDMS) part of the chip, and the other is the coverslip. In this work, the production process of this chip is improved. The functionality of these chips and the time dependence of the age of the chip and the polydimethylsiloxane from which the chips are made were tested using fluorescence microscopy.
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