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Molekulární epidemiologie druhů Crithidia mellificae a Lotmaria passim v populaci včelstev
 VOČADLOVÁ, Kateřina
The increased honey bee colony losses in the last decade gain a considerable attention of scientists and public. The causes of these losses include a wide range of biotic and abiotic factors, but pathogens and parasites are probably the main ones. Monoxenous trypanosomatids in honey bee gut Lotmaria passim and Crithidia mellificae were neglected for a long time but according to recent studies they seem to participate in those colony declines. Trypanosomatids are widespread parasites, including also the causes of some human illnesses, such as sleeping sickness, Chagas disease and leishmaniasis. The mechanism of the effect on honey bee health is not well understood so far. The aim of this thesis was to verify the occurrence of the trypanosomatids in honey bee samples from two regions in Czech Republic. The methods, based on detection of specific DNA loci, confirm the high prevalence of L. passim, which was founded in majority (71 %) of the samples. On the contrary, no samples were positive to C. mellificae.
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Heat treatment of milk and its importance
 VOČADLOVÁ, Kateřina
The aim of this work is to assess the public knowledge of heat treatment, its purpose and effect on milk quality and to find out their attitude to raw milk consumption. Heat treatment of milk is a commonly used part of dairy processing. Although the heat treatment has a significant impact on ensuring microbial quality, safety and shelf life of milk, its application still face a public disagreement, primary in connection with potential health benefits, which are lost during heat treatment. The survey took place (from November 2015 to February 2016) in a randomly selected group of respondents (n=265). It was found that 22 % of respondents buy raw (unpasteurized) milk and only 27 % of them reported pasteurizing of the milk before consumption. 68 % of respondents just cool the milk down and consume it without any thermal treatment. 23 % of respondents though that pasteurization has negative effects on milk quality and 21 % of respondents said that consumption of milk pose no health risk.
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4 Vočadlová, Klára
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