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Study of the Effect of Flubendazole on the Glioblastoma Multiforme Cells In Vitro and In Vivo
Vítovcová, Barbora ; Rudolf, Emil (advisor) ; Slaninová, Iva (referee) ; Jendelová, Pavla (referee)
Study of the effect of flubendazole on the glioblastoma multiforme cells in vitro and in vivo Glioblastoma multiforme (GBM) belongs to one of the most common and most aggressive primary brain tumours in adults. Current therapeutic strategy is insufficient, and patient's prognosis is unfavourable, therefore prompting an intensive effort to improve therapeutic options of GBM. Present research focuses mainly on the identification of the new possible targets for therapy or on the optimalization of already existing treatment strategies in GBM. Highly discussed approach to GBM therapy is microtubule targeting, which is one of the fundamental ways to tumour treatment. For this purpose, chemotherapeutics already in current common practice or drugs originally intended for a different indication, such as flubendazole (FLU), originally human and veterinary anthelmintic, could be used. In previous studies FLU has already demonstrated an inhibitory effect on cells of various solid tumours and haematological malignancies through a specific interaction with microtubules. The aim of this work was to evaluate the inhibitory effect of FLU in different in vitro and in vivo GBM models. Study of FLU effect was performed in vitro using stabilized GBM cell lines (A172, T98G and U118MG) and GBM primary cultures derived from...
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The effect of flubendazole on the efficacy of paclitaxel in brest cancer cells
Vítovcová, Barbora ; Skálová, Lenka (advisor) ; Ambrož, Martin (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Barbora Vítovcová Supervisor: prof. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: The effect of flubendazole on the efficacy of paclitaxel in breast cancer cells Flubendazole (FLU) is a common anthelmintic drug for veterinary and human use. Its molecular mechanism of action is based on binding to β-tubulin subunit of microtubules. FLU was previously identified to have antitumor activity. The aim of this study was to evaluate the effect of FLU on antiproliferative efficacy of paclitaxel (PTX) in breast cancer cells. For this study, three breast cancer cell lines were used - non-metastatic line MCF-7 and two metastatic cell lines, MDA-MB-231 and BT-474. The effects of FLU, PTX and FLU + PTX combination on viability of the cells (determined using WST-1 analysis) and on the expression of molecules involved in cell adhesion, migration and proliferation on mRNA (RT-PCR analysis) and protein levels (western blotting) were studied and compared. The activity of caspases after PTX and FLU treatment was evaluated using luminescence measurement. FLU has significant antiproliferative effect in all tested cell lines. MCF-7 and MDA-MB-231 have been shown as the most sensitive to PTX and FLU treatment....
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The effect of flubendazole on the efficacy of paclitaxel in brest cancer cells
Vítovcová, Barbora ; Skálová, Lenka (advisor) ; Ambrož, Martin (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Barbora Vítovcová Supervisor: prof. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: The effect of flubendazole on the efficacy of paclitaxel in breast cancer cells Flubendazole (FLU) is a common anthelmintic drug for veterinary and human use. Its molecular mechanism of action is based on binding to β-tubulin subunit of microtubules. FLU was previously identified to have antitumor activity. The aim of this study was to evaluate the effect of FLU on antiproliferative efficacy of paclitaxel (PTX) in breast cancer cells. For this study, three breast cancer cell lines were used - non-metastatic line MCF-7 and two metastatic cell lines, MDA-MB-231 and BT-474. The effects of FLU, PTX and FLU + PTX combination on viability of the cells (determined using WST-1 analysis) and on the expression of molecules involved in cell adhesion, migration and proliferation on mRNA (RT-PCR analysis) and protein levels (western blotting) were studied and compared. The activity of caspases after PTX and FLU treatment was evaluated using luminescence measurement. FLU has significant antiproliferative effect in all tested cell lines. MCF-7 and MDA-MB-231 have been shown as the most sensitive to PTX and FLU treatment....
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The effect of flubendazole on the efficacy of paclitaxel in brest cancer cells
Vítovcová, Barbora ; Skálová, Lenka (advisor) ; Ambrož, Martin (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Barbora Vítovcová Supervisor: prof. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: The effect of flubendazole on the efficacy of paclitaxel in breast cancer cells Flubendazole (FLU) is a common anthelmintic drug for veterinary and human use. Its molecular mechanism of action is based on binding to β-tubulin subunit of microtubules. FLU was previously identified to have antitumor activity. The aim of this study was to evaluate the effect of FLU on antiproliferative efficacy of paclitaxel (PTX) in breast cancer cells. For this study, three breast cancer cell lines were used - non-metastatic line MCF-7 and two metastatic cell lines, MDA-MB-231 and BT-474. The effects of FLU, PTX and FLU + PTX combination on viability of the cells (determined using WST-1 analysis) and on the expression of molecules involved in cell adhesion, migration and proliferation on mRNA (RT-PCR analysis) and protein levels (western blotting) were studied and compared. The activity of caspases after PTX and FLU treatment was evaluated using luminescence measurement. FLU has significant antiproliferative effect in all tested cell lines. MCF-7 and MDA-MB-231 have been shown as the most sensitive to PTX and FLU treatment....
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Problems of left-handed at physiotherapy
VÍTOVCOVÁ, Barbora
The topic of my thesis is "Problems of left-handed in physiotherapy". The theoretical part deals with laterality, dominance and hemispheric stroke itself. Further I am describing hemispheric stroke from the point of view of physiotherapist, where I'm interested in the developmental stages of stroke rehabilitation therapy, the procedure for examining the patient and physiotherapy methods, which can be used in therapy. The theoretical part is dealing with occupational therapy and assistive devices. The practical part is a qualitative method. For the preparation of the input and output tests I used the history data, patient interview, medical records and kinesiology tests. The research was done at retirement home Máj in České Budějovice. The research sample consisted of two patients in the subacute stage CMP. One patient was left-handed and had affected the right hemisphere dominant left-sided hemiparesis with. The second patient was right-handed and had affected the left hemisphere dominant with right-sided hemiparesis. Physiotherapy lasted for two months (January, February 2016) 1× weekly. When I could not come, the patients had the therapy with physiotherapists, who normally work there. The effectiveness of therapy was based on the results of input and output tests. The main goal was to find out, if it is possible, to create eaqual conditions for inclusion in ordinary daily life of patients after stroke. There is a left-handed patient with left hemiparesis when there is affected right dominant hemisphere and right-handed patient with right-sided hemiparesis, which has affected his left dominant hemisphere. I evaluate which of the patients will heal faster and better. Comparing the therapy of each of them aims to determine whether all healing techniques, occupational therapy and physiotherapy equipment adapted to the situation where is affection of right or left half of the body. The results of the research part of the thesis agree with the statement Josef-Hermann Zoche (2006), who says in his book, that the lefties after a stroke recover faster and better than right-handed. But I think, that this statement can not be considered completely truthful and objective, because there are many factors that can influence retirement. From my resoluts it is also clear, that there was an improvement of both of the patients. It was also confirmed, that the early threatment and rehabilitation is very important. Also cooperation of medical stuff, age, motivation and cooperation of the patient, support from family and friends are necessary. During therapy, I have not noticed any difference in treatment techniques that I used, aids of occupational therapy or physiotherapy procedures in connection with laterality. I believe that there is no significant difference in supply assistive devices for right-handed and left-handed patients. Only aids for left-handers are more expensive and less available.
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