|
|
Digital Literacy Among Teachers of Learners with Disabilities in Czech Republic and Kenya
Mwongela Kavua, Martin ; Šiška, Jan (advisor) ; Pančocha, Karel (referee) ; Tichá, Renáta (referee)
The study focused on establishing how teachers use their digital literacy skills to facilitate learning in Kenya and the Czech Republic. This was achieved by focusing on four objectives: to identify how teachers apply digital literacy skills to facilitate learning; to establish self- efficacy in digital literacy among primary school teachers of learners with disabilities in Kenya and the Czech Republic; to investigate the challenges to the application of digital competencies in class by teachers of learners with disabilities in special primary schools; and to explore how teachers of learners with disabilities overcome the challenges faced in the application of digital competencies. The study was guided by the Self-efficacy element of Albert Bandura's Socio- Cognitive Theory. A descriptive research design was used for the study. The target population for the study was teachers of learners with disabilities in primary schools in Kenya and the Czech Republic. Probabilistic sampling was applied in the study. A semi-structured questionnaire and observation checklist were used to collect data from 456 teachers: 329 from Kenya and 127 from the Czech Republic. A mixed-method approach was used in the analysis, where statistical methods including descriptive, independent sample and chi-square tests were...
|
|
|
Flow injection analysis of selected glycosaminoglycans with spectrofluorimetric detection
Tichá, Renata ; Nesměrák, Karel (advisor) ; Rychlovský, Petr (referee)
The thesis is focused on a determination of heparin and chondroitin sulfate, using flow injection analysis with spectrofluorimetric detection. The determination is based on the interaction of negatively charged heparin, chondroitin sulfate resp., with a cationic dye (azure B or phenosafranine) which is manifested by the decrease in fluorescence intensity of the dye in its emission maximum. The optimal conditions for the determination in static mode were found, and calibration dependencies were measured. The conditions of FIA were optimized and following parameters were established: the volume of dispensed sample of 100 ml, the length of the reaction coil 60 cm, the flow rate 0.7 ml min-1 , the concentration of azure B 1.6×10-5 mol dm-3 , the concentration of phenosafranine 3.5×10-5 mol dm-3 . For the determination of heparin using azure B it was found: LOD = 0.023 IU ml-1 , LOQ = 0.186 IU ml-1 , and linear dynamic range 0.19-1.43 IU ml-1 . For the determination of heparin using phenosafranine it was found: LOD = 0.102 IU ml-1 , LOQ = 0.192 IU ml-1 , and linear dynamic range 0.19-1.79 IU ml-1 . For the determination of chondroitin sulfate using azure B it was found: LOD = 0.58 mg dm-3 , LOQ = 2.37 mg dm-3 , and linear dynamic range 2.37-8.32 mg dm-3 . The developed determination was applied to the...
|
|
| |
|
|
Flow injection analysis of selected glycosaminoglycans with spectrofluorimetric detection
Tichá, Renata ; Nesměrák, Karel (advisor) ; Rychlovský, Petr (referee)
The thesis is focused on a determination of heparin and chondroitin sulfate, using flow injection analysis with spectrofluorimetric detection. The determination is based on the interaction of negatively charged heparin, chondroitin sulfate resp., with a cationic dye (azure B or phenosafranine) which is manifested by the decrease in fluorescence intensity of the dye in its emission maximum. The optimal conditions for the determination in static mode were found, and calibration dependencies were measured. The conditions of FIA were optimized and following parameters were established: the volume of dispensed sample of 100 ml, the length of the reaction coil 60 cm, the flow rate 0.7 ml min-1 , the concentration of azure B 1.6×10-5 mol dm-3 , the concentration of phenosafranine 3.5×10-5 mol dm-3 . For the determination of heparin using azure B it was found: LOD = 0.023 IU ml-1 , LOQ = 0.186 IU ml-1 , and linear dynamic range 0.19-1.43 IU ml-1 . For the determination of heparin using phenosafranine it was found: LOD = 0.102 IU ml-1 , LOQ = 0.192 IU ml-1 , and linear dynamic range 0.19-1.79 IU ml-1 . For the determination of chondroitin sulfate using azure B it was found: LOD = 0.58 mg dm-3 , LOQ = 2.37 mg dm-3 , and linear dynamic range 2.37-8.32 mg dm-3 . The developed determination was applied to the...
|
|
| |
|
| |
|
| |
|
| |
guest ::
RSS feed.