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Retention Behaviour of Catecholamines and Their Precursors under HILIC Conditions
Tibenský, Andrej ; Kastner, Petr (advisor) ; Štěrbová, Petra (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Andrej Tibenský Supervisor: PharmDr. Petr Kastner, Ph.D. Title of thesis: Retention behaviour of catecholamines and their precursors under HILIC conditions The aim of this diploma thesis was to study retention behaviour of catecholamines and their precursors under Hydrophilic Interaction Liquid Chromatography by using spectrophotometric and fluorometric detection. 5 analytes were examined - adrenaline, noradrenaline, dopamine, L-DOPA, and tyrosine. Retention behaviour studying began by using LUNA 3 µm (PFP)2 column which possesses both RPLC and HILIC characteristics with 2 different mobile phases. The first one consisted of 25 mM acetic acid in mixture of acetonitrile and water and the second mobile phase was 25 mM acetate buffer (pH 4,50) in mixture of acetonitrile and water. Subsequently, the effect of stationary phase was studied on neutral column XBridge BEH Amide and columns Avantor ACE HILIC-A and Sequant ZIC-HILIC with ionic interactions. Mobile phases consisted of high proportion of acetonitrile (70-90 %) and their composition was the following - 25 mM acetic acid in mixture of acetonitrile and water. Then, the effect of acetic acid concentration as a mobile...
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Retention Behaviour of Catecholamines and Their Precursors under HILIC Conditions
Tibenský, Andrej ; Kastner, Petr (advisor) ; Štěrbová, Petra (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Andrej Tibenský Supervisor: PharmDr. Petr Kastner, Ph.D. Title of thesis: Retention behaviour of catecholamines and their precursors under HILIC conditions The aim of this diploma thesis was to study retention behaviour of catecholamines and their precursors under Hydrophilic Interaction Liquid Chromatography by using spectrophotometric and fluorometric detection. 5 analytes were examined - adrenaline, noradrenaline, dopamine, L-DOPA, and tyrosine. Retention behaviour studying began by using LUNA 3 µm (PFP)2 column which possesses both RPLC and HILIC characteristics with 2 different mobile phases. The first one consisted of 25 mM acetic acid in mixture of acetonitrile and water and the second mobile phase was 25 mM acetate buffer (pH 4,50) in mixture of acetonitrile and water. Subsequently, the effect of stationary phase was studied on neutral column XBridge BEH Amide and columns Avantor ACE HILIC-A and Sequant ZIC-HILIC with ionic interactions. Mobile phases consisted of high proportion of acetonitrile (70-90 %) and their composition was the following - 25 mM acetic acid in mixture of acetonitrile and water. Then, the effect of acetic acid concentration as a mobile...
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