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Nový polymorfizmus genu apolipoprotein A2 a jeho asociace s obsahem mastných kyselin u prasat
Sukhov, Oleg
This thesis studies the problematic of the new polymorphism APOA2 gene and that association with fatty acids contain in a group of Czech Large White pigs. APOA2 gene (ID: 100153243) is a candidate gene for porcine meat quality. The aim of thesis was to analyze the influence of selected polymorphisms on fatty acids and intramuscular fat contain. Among fatty acids was observed a contain of: tetradecenoic acid, palmitic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid, linolenic acid, arachidonic acid, arachidic acid a eicosapentaenoic acid (EPA). Have been used molecular-genetic methods such as primers design in the OLIGO software, PCR, gel electrophoresis a sequencing by Sanger method. The results were processed by form of genotype frequency and followed by associative analysis with a mixed linear model. The values of the relative alleles frequency of polymorphism APOA2 T>A rs80803879 were as follows: A = 0,086, T = 0,914 and relative alleles frequency of APOA2 G>A rs331415849: A = 0,068 a G = 0,948. Polymorphism associations were found for fatty acids: myristoleic acid, acid palmitoleic acid, oleic acid, arachidonic acid, and arachidic acid.
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Vliv ročního období a fáze laktace na potravní chování holštýnských krav ve vybraném chovu
Sukhov, Oleg
The aim of this thesis was to evaluate changes in food behavior cows of Holstein cattle during the year and the stage of lactation in a selected breeding. Monitored behaviors were: feed intake, water intake, rest, standing (especially in the hallways), movement and defecation. Ethological observations cows in the barn covered the period of one calendar year (may -- december 2014). In every season there were two-hour monitoring. Speeches animals were recorded every 3 minutes to ethogram. Records of monitoring were subsequently entered into ethogram and processed according to conventional statistical methods in MS Excel. Take aim was to evaluate own results and then compare them with the opinions of the authors reported. The obtained results can be determined considerable variation in the behavior of cows, which were affected by the current season: In the summer, there were frequent cows standing in the corridors, boos and increased fluid intake. Subsequently, in relation to water intake appeared decrease in feed intake. Conversely, in the winter, an increased incidence of lying and feed intake (compared to the summer), it is even behavior components which have the greatest impact on milk production.
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