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National Repository of Grey Literature 3 records found  Search took 0.01 seconds. 
Testing of a new contactless conductivity detector for capillary electrophoresis
 Srchová, Zuzana ; Coufal, Pavel (advisor) ; Křížek, Tomáš (referee)
This work deals with testing of basic parameters of a contactless conductivity detector for capillary electrophoresis. The aim was to find area where the resulting signal dependence on concentration is linear, to determine the threshold sensitivity of the detector and verify that the detector is capable of providing reproducible results.
Detailed record
Analysis of MRCK alpha protein by HPLC
 Srchová, Zuzana ; Coufal, Pavel (advisor) ; Hudeček, Jiří (referee)
The aim of this diploma thesis was to analyze and then obtain MRCK alpha protein from biological samples. For the better visualization is protein kinase fused with green fluorescent protein (GFP), which fluoresces green light, when irradiated with ultraviolet light. Individual measurements were implemented by using the HPLC apparatus with UV and fluorescence detection. A necessary part of the implementation of a series of acts dealing with the characterization of available biological samples, which differ in their content from each other. The aim was to test a wide range of different columns, working on the principle of reverse- phase chromatography, ion exchange chromatography and gel permeation chromatography. Furthermore, it was necessary to find a suitable mobile phase and optimize the measuring conditions for the separation of proteins. Helpful in the identification of compounds present in the samples became mass spectrometry.
Detailed record
Testing of a new contactless conductivity detector for capillary electrophoresis
 Srchová, Zuzana ; Coufal, Pavel (advisor) ; Křížek, Tomáš (referee)
This work deals with testing of basic parameters of a contactless conductivity detector for capillary electrophoresis. The aim was to find area where the resulting signal dependence on concentration is linear, to determine the threshold sensitivity of the detector and verify that the detector is capable of providing reproducible results.
Detailed record

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