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In vitro cultures of medicinal plants - XV
Slavík, Marek ; Tůmová, Lenka (advisor) ; Martin, Jan (referee)
The subject of this study is the evaluation of secondary metabolites production in Hypericum perforatum L. cultures in vitro after elicitor treatment. The aim was to find if orthosilicic acid as abiotic elicitor increases the flavonoid and hypericin production in Hypericum perforatum L. cultures in vitro. Experiment was carried out in callus and suspension cultures of H. perforatum using Murashige - Skoog nutrient medium78 supplemented with 10 mg. ml-1 α-naphtylacetic acid as growth regulator. The elicitor was added in the form of solution in 3 different concentrations (C1 = 10.4047∙10-3 mol l-1 , C2 = 10.4047∙10-4 mol l-1 , C3 = 10.4047∙10-5 mol l-1 ), it was affecting 6, 12, 24, 48, 72 and 168 hours. The content of flavonoids and hypericin was determined by HPLC. Secondary metabolites release into nutrient medium was also a part of this study. The increasing flavonoid and hypericin production in callus cultures after elicitor application at any concentrations was not observed. The maximum flavonoid content (0.04 mg g-1 DW) in suspension culture was detected after 72 h of elicitor treatment in concentration of C1 where the maximum hyperoside production was observed. The maximum hypericin production (0.21 mg g-1 DW) in suspension culture was detected after 12 h of elicitor application in...
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Foreign trade with ICT goods in the Czech Republic
Slavík, Marek
The thesis examines quantitative development of foreign trade with ICT goods in the Czech Republic since establishment of the republic in 1993. For this purpose, original data generated from the external trade database gathered by the Czech Statistical Office are used. The main goal of the thesis is to perform thorough de-scription of development of foreign trade with ICT goods in the Czech Republic. Among others, the analysis includes trend and seasonality description, evaluation of impact of the economic crisis in 2008, forecasting of development for 2015, 2016 and 2017, and basic international comparison with several members of the European Union.
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