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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Characterization of polar tube proteins of microsporidia.
 Satrapová, Alexandra ; Doležal, Pavel (advisor) ; Štáfková, Jitka (referee)
Microsporidia is a group of intracellular parasites belonging to Fungi. Even though this group seems to be diverse with great variety of hosts and infected tissues they share completely unique invasion apparatus. A key organelle of this apparatus called the polar tube serves as a conduit for sporoplasm passage into the host cell. It consists of PTP proteins which are the main interest of this diploma thesis. Major effort was devoted to the study of the two main PTP proteins. In contrast to already published data, we were unable to verify the interaction of PTP proteins using a yeast two-hybrid system. On the contrary, experiments with in vitro prepared proteins have shown the possible formation of homopolymeric complexes of PTP2 and PTP3 proteins from Nosema bombycis (NbPTP2, NbPTP3). Several constructs were prepared for the expression of recombinant NbPTP2 and NbPTP3 proteins in Escherichia coli. Expression of NbPTP2 was successful but under native conditions the protein wasn't obtained in soluble fraction allowing its subsequent purification. The preparation of recombinant NbPTP3 was complicated by the rapid formation of presumably degradation products. Therefore, a final construct was made to allow purification of intact NbPTP3 using two purification tags at the N- and C-termini of the protein....
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Nosema bombycis as a model organism for polar tube study.
 Satrapová, Alexandra ; Petrů, Markéta (advisor) ; Trdá, Lucie (referee)
Microsporidia is a group of obligatory intracellular parasites with a unique invasion mechanism. One of the highly specialized invasion structures is the polar tube which is an example of a biological nanotube the study of which could bring usable knowledge in biotechnology. The thesis is a literary overeview which deals with the structure of microsporidian spores and presents N. bombycis as a suitable representative for the study of the infectious apparatus. In more detail, it summarizes the background to the structure of the polar tube and wall of N. bombycis spore.
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3 Satrapová, Anna
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