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Exprese transgenu bphC u ječmene
Sedlářová, Kateřina
Polychlorinated biphenyls are toxic organic compounds, characterized by a low degradability in the environment and accumulation in the food chain. This is the reason, why decontamination of soils polluted with such substances is very important issue for today's society. Decontamination of areas infected by PCBs is very complicated due to its physical and chemical characteristics. Appropriate decontamination method is phytoremediation, which is used for removing polychlorinated biphenyls by natural processes of plants. Using the transformation of plants can increase the efficiency of phytoremediation. The thesis has been investigated in expression of remediation gene bphC/His at T2 generation of spring barley (Hordeum vulgare L.), variety Golden Promise at the DNA, RNA and protein levels. Expression of the transgene was detected using PCR, real-time PCR and Western blot. To obtain dihaploid homozygous plants of spring barley, variety Golden Promise containing a phytoremediation gene bphC/His was used anther cultures technique. In the thesis were choosed plants from the T2 generation, showing the presence of the inserted gene to test the ability of transgenic plants to accumulate polychlorinated biphenyls.
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Special tools for teaching German for pupils with specific of learning in secondary schools.
SEDLÁŘOVÁ, Kateřina
The theoretical part of the dissertation describes some specific learning disabilities (next SLD) - especially dyslexia, pays attention to causes of the defects, diagnosis and reeducation. Its aim is to describe the personality of these children or their perception of colours. Further, some possibilities how to work with a child with a learning disability in foreign language lessons at basic schools are discussed. The practical part is aimed at teaching aids production. It means a textbook, a CD, sheets with appendices, etc. Each aid is described and its benefit for both, the pupil and the teacher, are mentioned and also teaching methods are explained.
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