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Analýza vybraných zemědělských produktů z hlediska optimálního poměru n-3 a n-6 polynenasycených mastných kyselin
Rozíková, Veronika
The practical part of this thesis consists of free main chapters: the method for the determination of polyunsaturated fatty acids by gas chromatography; the effect of addition of Spanish Sage seeds on the content of PUFA n-3 in the tissues of animals; the effect of EPA and DHA content of blood plasma lipids and fatty acid in the tissues of rats. The aim was to increase the content of PUFA n-3 in animal tissues improving the ratio of n-6/n-3 fatty acids, and reduced levels of total cholesterol and LDL cholesterol levels and to raise HDL cholesterol. The experimental part of this work was supported by the IGA. The effect of addition of Spanish Sage seeds on the content of n-3 PUFA in the tissues of animals was analyzed in quail eggs, chicken meat and free species of insects to qualitative and quantitative analysis of fatty acids.The content of PUFA n-3 in animal tissues was increased in groups with addition of Spanish Sage seeds in the diet (P < 0,05). The addition of Spanish sage seeds to feed the animals had an impact on the sensory properties of their products. The effect of EPA and DHA content of blood lipid levels and fatty acids composition in rat tissues was achieved by the addition of salmon and palm oil to feed animals. The group with the addition of salmon oil in the diet had lowered total cholesterol, LDL cholesterol and triglycerides as compared to the control group and the group with the addition of palm fat in feed (P < 0,05).

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