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Influence of experimental intestinal dysbiosis on behavioral, neuroendocrine and immune properties of mice and the possibility of modifying it by administration of probiotic strain Escherichia coli O83:K24:H31
Avramová, Pavla ; Černý, Viktor (advisor) ; Roubalová, Radka (referee)
The gut microbiota is a key factor influencing the function of many organs in the human body, including the nervous, immune, and endocrine systems. This dynamic ecosystem can be modulated by diet, age, environment, even medication. The balanced composition of the microbiota is crucial for individual health, and its disruption leads to dysbiosis. The connection between the gut and the central nervous system is mediated by the microbiota-gut- brain axis. This axis consists of several pathways interconnected to mutually regulate their functions. Among the major components of the axis are the neural pathway, led by the vagus nerve, the immune pathway, and the endocrine pathway, including the HPA axis. Recent studies suggest that probiotic bacteria have the ability to partially rectify dysbiosis and alleviate its consequences for the organism. This thesis focuses on researching the impact of gastrointestinal tract microbiota on the behaviour, immunity, and neuroendocrine aspects of mice. It delves into experimentally inducing dysbiosis using antibiotics and subsequently addressing it with the probiotic bacterium Escherichia coli O83:K24:H31. The aim is to determine whether these probiotics can mitigate the adverse effects of dysbiosis on the immune and neuroendocrine systems, as well as behavioural...
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Gut microbiome and autoimmune mechanisms in patients with type 1 narcolepsy
Ježková, Janet ; Roubalová, Radka (advisor) ; Hrdý, Jiří (referee)
Narcolepsy Type 1 (NT1) is a chronic neurological disease characterized by the presence of cataplexy and excessive daytime sleepiness. NT1 probably arises from autoimmune destruction of orexinergic neurons in the hypothalamus. These neurons are the only known producers of the neuropeptides orexin A and B, which are involved in the regulation of sleep and wakefulness. NT1 is often accompanied by comorbidities such as obesity, type 2 diabetes, hormonal disorders, depression, and anxiety. Gut microbiota affects the quality of sleep by the production of various metabolites. It is considered that it may be involved in the pathogenesis of NT1 or in the development of related comorbidities. In our study, we analyzed the gut microbiota composition of 41 NT1 patients and 32 healthy controls using next-generation sequencing. The diversity of patients' gut microbiota did not differ significantly from healthy controls. In patients, we revealed a positive correlation between the abundance of the bacterial family Coriobacteriaceae, BMI, and the disease duration. Besides, we observed a negative correlation between the Coriobacteriaceae family and cholesterol levels, suggesting that these bacteria are involved in host lipid metabolism. Compared to healthy controls, a higher abundance of bacteria from the families...
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