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Screening
Richter, Lucie ; Šafratová, Marcela (advisor) ; Cahlíková, Lucie (referee)
IX. ABSTRACT Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of: Pharmacognosy and Pharmaceutical Botany Candidate: MUDr. et Mgr. Lucie Richter Consultant: PharmDr. Marcela Šafratová, Ph.D. Title of Thesis: SCREENING STUDY OF SEVERAL PLANT SPECIES AND THEIR EFFECT ON HUMAN ENZYMATIC SYSTEMSII. Five tropical plants (Geissospermum vellosii stembark, Calotropis gigantea aerial part, Cissampelos pareira stem, Virola sebifera stembark and Virola surinamensis stembark) were screened for alkaloid presence and a preliminary estimate of their potential as anticholinesterase or cytotoxic agent was performed. Ethanolic extracts were prepared from these plants and further processed into the ethyl acetate alkaloid extracts, which were then analysed using the GC/MS method. For extracts with the confirmed presence of alkaloids (Geissospermum vellosii, Calotropis gigantea and Virola sebifera) by TLC including UV detection and Dragendorff test, the anticholinesterase activity against human AChE and BuChE was measured employing Ellman method and compared to standards (galantamine, huperzine A and rivastigmine). Cytotoxic activity of these extracts was also evaluated on Jurkat, MOLT-4, A549, HT-29, PANC-1, A2780, HeLa, MCF-7, SAOS-2 tumour cell lines and MRC-5 control cell line using MTT method...
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