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Vliv biologického a chemického ošetření na výskyt biogenních aminů u vojtěškových siláží
Remerová, Pavla
In the experiment, the influence of two different biological silage additives (Lactococcus lactis (NCIMB 30117), Lactobacillus plantarum (DSM 16568), Enterococcus faecium (DSM 22 502 / NCIMB 11181), the enzyme xylanase EC 3.2.1.8.) And chemical (43% formic acid 30% ammonium formate and 10% propionic acid and 2% benzoic acid) at a concentration of biogenic amines, nutrient content, evaluation of the quality of the fermentation process and microbiological parameters for alfalfa silage (Medicago sativa). Silage fermentation time was 90 days. Biological silage additive significantly increased incidence of putrescine (51%; P <0.05), lactic acid (11%; P <0.05), protein (11%; P <0.05). Conversely, there was a significant reduction cadaverine (29%; P <0.05), histamine (57%; P <0.05), spermidine (15%; P <0.05), spermine (55%; P <0.05), acid acid (40%; P <0.05), ethanol (55%; P <0.05) ammonia (25%; P <0.05), and ash (9%; P <0.05). In the group treated by chemical silage additive is statistically significantly increased histamine content (63%; P <0.05) and tyramine (34%; P <0.05). Decreases in the concentration of putrescine (18%; P <0.05), cadaverine (55%; P <0.05), spermidine (47%, P <0.05), spermine (45%; P <0.05), lactic acid ( 16%; P <0.05), acetic acid (46%; P <0.05) ammonia (59%, P <0.05), ash (13%, P <0.05) and fat (24%, P < 0.05). From a microbiological point of view have been affected by the lactic acid bacteria, molds, yeasts, enterobacteria and total number of microorganisms. The results show that to maintain hygiene and healthy silage is preferable to use biological and chemical silage additives. For alfalfa silage inoculant chemical has higher efficiency in maintaining health security silage compared with biological inoculant
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Záchrana genofondu vybraných starých odrůd růží z areálu Flora Olomouc množením in vitro
Remerová, Pavla
In the thesis "Saving the gene pool of selected varieties of old roses from the area Flora Olomouc using in vitro propagation" are listed procedures for sampling explants and primary cultures foundation varieties of roses Klimentina, Jan Palach, Fortissimo, Pastorale and Julius Fabiancs de Misefa. It was developed a protocol in vitro propagation of roses and selected media and culture conditions for the maintenance of multiplication in gene bank. Varieties Klimentina, Jan Palach, Fortissimo, Pastorale and Julius Fabiancs de Misefa formed its foundation. An integral part of the above results indicate that the formation of roots and convert rooted plants in non-sterile conditions. Finally, we tested the stability of donor plants and plant tissue using SSR primers in the varieties Klimentina, Jan Palach, Fortissimo and Julius Fabiancs de Misefa.
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