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Bacterial interactions and secondary metabolites in plant and soil environment
Rapoport, Daria ; Marečková, Markéta (advisor) ; Lovecká, Petra (referee) ; Brabcová, Vendula (referee)
Phylum Actinobacteriota represents one of the major phyla in plant and soil environments. Known for the ability to produce numerous secondary metabolites, Actinobacteriota may affect other bacteria and plants in various ways. The thesis aimed at assessing the connection between the production of secondary metabolites and interactions of actinobacteria in plant and soil environments. In the first part, we assessed how rare actinobacteria from acid soil affect cultivable soil bacteria. We isolated a collection of actinobacteria from acid soil dominated by previously uncultivable lineage and isolated a representative described as a new family Treboniaceae and multiple other potentially novel species of known genera. The genome and metabolome analysis of Trebonia kvetii, sp.nov. gen.nov., demonstrated its ability to produce unusual and potentially novel bioactive metabolites. We designed an experiment where a single strain was co-cultivated with the total soil bacterial community of the same soil in a set up, where a free exchange of diffusible metabolites was allowed between them. Three actinobacteria strains coming from distinct lineages were selected for this interaction. Each strain significantly and specifically affected cultivable bacteria as well as the metabolite pool, part of which was induced...
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Development of a tool for digital terrain analysis
Rapoport, Daria ; Barták, Vojtěch (advisor) ; Bašta, Petr (referee)
Component of many hydrological applications using digital terrain models is the calculation of flow accumulation and specific catchment area, which are broadly used conceptions in hydrological modelling.
The goal of this diploma thesis was a proposal of tools for calculation of flow accumulation and specific catchment area by Multiple Flow Direction (MFD) algorithm as more appropriate for many hydrological applications then Single Flow Direction algorithm built-in in ArcGIS. The toolset should serve as an educational and research tool at the Faculty of Environmental Sciences, CULS, but not only there.
The theoretical part of the thesis gives examples of digital terrain models applications in general and in hydrology, summarizes basic methods that define flow direction and flow accumulation of surface runoff in grid-based terrain models and their significance for hydrological modelling. Selected methods and their implementations are described in details in methodological part of the thesis. The tools were created in Python language and may be used in ArcGIS interface.
The functionality of the toolset was tested on a coarse-resolution hydrologically correct digital terrain model of basin Moravian Dyje and simulation results has been extensively compared to those produced by Single Flow direction algorithm.
It is suggested that created toolset would be developed further in direction of calculation of additional related terrain attributes (e.g.Topographic Wetness Index), river network extraction with MFD, dealing with flat and sink areas in input DEM, and other flow direction algorithms implementation.
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