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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
The study of expression of various markers of aterogenesis in apoE/LDLr-deficient.
 Rambousková, Kristýna ; Nachtigal, Petr (advisor) ; Štaud, František (referee)
The aim of this diploma thesis was to set immunohistochemical detection of nuclear transcription factor kappaB (NF-κB). We studied its expression in aortic sinus in 8 and 16 weeks old apoE/LDL receptor deficient mice. Female apoE/LDL receptor mice were divided into two groups. The first group (n=8) was represented by eight weeks old mice. In the 16 weeks group (n=8) mice were fed with cholesterol enriched diet (0.15% of cholesterol) for the period of eight weeks. Indirect fluorescence immunohistochemistry with detection of antibody reaction with CY3 red fluorochrome was used for the detection of active form of NF-κB. In 8 weeks old mice the NF-κB expression was detected in endothelial cells and in some smooth muscle cells in vessel media. In 16 weeks old mice the NF-κB expression was detected inside atherosclerotic plaques and in vessel media under these plaques predominantly by macrophages and smooth muscle cells. In conclusion, the results of immunohistochemical analysis showed induction of inflammatory reaction by various cells during atherogenesis in blood vessels of apoE/LDL receptor deficient mice which will be used in prospective studies.
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The study of expression of various markers of aterogenesis in apoE/LDLr-deficient.
 Rambousková, Kristýna ; Štaud, František (referee) ; Nachtigal, Petr (advisor)
The aim of this diploma thesis was to set immunohistochemical detection of nuclear transcription factor kappaB (NF-κB). We studied its expression in aortic sinus in 8 and 16 weeks old apoE/LDL receptor deficient mice. Female apoE/LDL receptor mice were divided into two groups. The first group (n=8) was represented by eight weeks old mice. In the 16 weeks group (n=8) mice were fed with cholesterol enriched diet (0.15% of cholesterol) for the period of eight weeks. Indirect fluorescence immunohistochemistry with detection of antibody reaction with CY3 red fluorochrome was used for the detection of active form of NF-κB. In 8 weeks old mice the NF-κB expression was detected in endothelial cells and in some smooth muscle cells in vessel media. In 16 weeks old mice the NF-κB expression was detected inside atherosclerotic plaques and in vessel media under these plaques predominantly by macrophages and smooth muscle cells. In conclusion, the results of immunohistochemical analysis showed induction of inflammatory reaction by various cells during atherogenesis in blood vessels of apoE/LDL receptor deficient mice which will be used in prospective studies.
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2 Rambousková, Kateřina
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