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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Příprava rekombinantních proteinů DS47 a IDGF3 v bakulovirovém expresním systému a jejich funkční testy na buňkách \kur{Drosophila melanogaster in vitro}
 ROUHOVÁ, Lenka
The aim of this work was the production of two Drosophila growth factor proteins, DS47 and IDGF3. The cDNAs encoding the two proteins were cloned into a baculovirus transfer vector and used for the recombination with bacmid form of baculovirus maintained in Escherichia coli. The recombinant baculoviral DNA was transfected to insect cells. Baculovirus- infected cells produced recombinant His-tagged proteins which were purified from growth media by nickel-agarose affinity chromatography. The function of recombinant proteins was verified by in-vitro growth/survival tests.
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The effect of IDGF2 protein on expression of selected genes in cell line Cl.8+
 ROUHOVÁ, Lenka
The imaginal discs growth factors (IDGFs) are proteins able to induce proliferation of Cl.8+ cells proliferation in collaboration with insulin. They are also connected with response to starvation stress and innate immunity. A genome-wide analysis of Cl.8+ cells treated with IDGF2, which was performed in our lab, detected tens of genes transcriptionally induced by this protein. The aim of this work was to confirm this effect with some of these genes using quantitative PCR.
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