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Evaluation of activity of potentional antifungal substances through the use of microdilution broth method II
Poučová, Michaela ; Křivčíková, Lucie (advisor) ; Vacková, Jana (referee)
Poučová Michaela Evaluation of activity of potentional antifungal substances through the use of microdilution broth method II Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Kralove Specialist in laboratory methods The main concern of this study was to test potential antifungal agents and evaluate their activities. During the experiment were tested two groups of compounds, derivatives pyrazin-2-carboxamide and salicylanilide. Using microdilution broth method were measured the activities of these substances against eight strains of filamentous fungi and yeasts, which included C. albicans, C. tropicalis, C. krusei, C. glabrata, T. beigelii, A. fumigatus, A. corymbifera and T. mentagrophytes. For determining the sensitivity of these strains of fungi against tested substances was prepared eleven different concentrations (500 mol∙l-1 , 250 mol∙l-1 , 125 mol∙l-1 , 62,5 mol∙l-1 , 31, 25 mol∙l-1 , 15,625 mol∙l-1 , 7,813 mol∙l-1 , 3,906 mol∙l-1 , 1,953 mol∙l-1 , 0,977 mol∙l-1 and 0,488 mol∙l-1 ), which let incubate with the fungi. Performing microdilution broth method was determined the minimum inhibitory concentration (MIC), whose value indicates the lowest concentration of test substance that causes visible suppression of growth of yeasts or filamentous fungi. Based on the...
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