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National Repository of Grey Literature

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Analysis of MHC-II bound peptides of dendritic cells Pavlosková, Jana ; Szotáková, Barbora (advisor) ; Balonová, Lucie (referee) Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Jana Pavlosková Supervisor: prof. Ing. Barbora Szotáková, Ph.D. Consultant: Ing. Marek Link, Ph.D. Title of diploma thesis: Analysis of MHC-II bound peptides of dendritic cells Antigen presentation is an important process for the immune system, which is mediated by antigen presenting cells. These cells can capture and process antigen material and present antigenic peptides to T lymphocytes. The immunopeptidome represents a collection of peptides displayed by MHC molecules on the surface of antigen presenting cells. Analysis of immunopeptidome plays a crucial role in identifying peptides to which the organism responds. This knowledge is important for developing new vaccines, medication, and disease diagnostics. This thesis focuses on the analysis of MHC-II bound peptides of dendritic cells, with the aim of verifying and demonstrating the suitability of methodological procedures for immunopeptidome analysis. The study was conducted on the mouse dendritic cells. Additionally, the cells were pulsed with the protein antigen ovalbumin to model foreign antigen binding and presentation. An immunoaffinity resin protein G agarose with immobilized antibody targeting I-Ab was successfully created for... Detailed record

Neurotransmitters in autism spectrum disorders Pavlosková, Jana ; Herink, Josef (advisor) ; Kovařík, Miroslav (referee) Detailed record

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