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National Repository of Grey Literature 1 records found  Search took 0.01 seconds. 
Development of HPLC method for the determination of amygdalin and serotonin in dietary supplement
 Parmová, Martina ; Chocholoušová Havlíková, Lucie (advisor) ; Aufartová, Jana (referee)
The analytical method for the determination of amygdalin and serotonin supplement B17 APRICARC using high-performance liquid chromatography was developed and validated. The 96 % ethanol was used for the extraction to obtain analytes. As a suitable chromatographic column was chosen the column Ascentis Express® OH5 (3 x 100 mm, 2,7 µm). The composition of the mobile phase for the analysis was 10 mM acetate buffer pH 3.8 : acetonitrile 10:90 (v:v). The flow rate was set on 0.8 mL / min, for the detection of amygdalin was used an UV detector (wavelength 215 nm) and for the detection of serotonin was used the fluorescence detector (Ex 280 nm , Em 340 nm ). A suitability test of chromatographic system was performed and these parameters were pursued: column efficiency, resolution peak symmetry factor and repeatability of analysis. Within the actual validation these parameters were pursued: accuracy, linearity, precision, and limit of detection and quantification.
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See also: similar author names
2 Parmová, Markéta
1 Parmová, Miroslava
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