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Determinating basal metabolic rate by indirect calorimetry method
POSTL, Roman
The topic of the bachelor thesis is "Determination of resting energy expenditure by indirect calorimetry". The method of indirect calorimetry is considered the gold standard for determining resting energy expenditure, which can further serve as a basis for setting optimal energy intake for the design of therapeutic diets, and is often compared with predictive equations, with which the determination of resting energy expenditure is simpler and less time-consuming. Data collection was performed by quantitative methods by measuring on indirect calorimetry, assessing dietary records and completed questionnaires, and obtaining anthropometric data. The research population consisted of 10 respondents approached through mass email correspondence or in person. The main aim of the study is to compare the results of the indirect calorimetry method with the recalculated results of the Harris-Benedict predictive equation on resting energy expenditure values. A sub-objective is to map the differences in measurement results based on the diet composition of the respondents. Together with this objective, the dissimilarity of energy intake versus calculated energy requirement is mapped, and lastly, the dissimilarity of measurement results due to respondents' body composition is mapped. As a result of the research, the deviation of +-10% between the measured and calculated values of resting energy expenditure is confirmed, which is also reported by authors of publications on a similar topic. Furthermore, the research shows a deviation of 18.34% between the recorded energy intake and energy requirement with a tendency to underestimate dietary intake. The study also shows relationships between the measurement results and the percentage of diet and body composition of the respondents. The relationships are based on linear trend lines, which indicate, first - an increase in measured values with a higher proportion of protein and carbohydrate in the diet and a higher proportion of lean body mass, and second - a decrease in measured values with a higher proportion of fat in the diet and a higher proportion of adipose tissue in the body.
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