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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Detekce a identifikace virů pomocí sekvenování nové generace (NGS)
 PODRÁBSKÁ, Kateřina
Next generation sequencing is a modern method applied in plant virology for sensitive detection of previously characterized and novel pathogens without any preceding knowledge of them. In this study three novel and two already described viruses were detected by de novo assembly of Illumina single-end reads ( Hi-Seq 2500 system) from total poly(A) enriched RNA of diseased red clover (Trifolium pratense) and indicator plant (Nicotiana occidentalis 37B). The complete genomic sequence of novel Red clover carlavirus A (RCCA) was determined from Illumina reads, 5´, 3´ RACE, cloning, RT-PCR and Sanger sequencing. The presence of RCCV was also confirmed in mechanically inoculated tobacco plant.
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Molecular analysis of the phytoplasma infecting (\kur{Primula acaulis})
 PODRÁBSKÁ, Kateřina
Phytoplasmas are the obligate intracellular wall-less bacteria infecting phloem of plants and are transmitted by the vectors from the class of Mollicutes. They are economically important pathogenes causing crop worldwide losses. The phytoplasma infecting Primula acaulis was detected and characterized by molecular methods in this thesis. The highly conserved 16S-23S ribosomal operon and more variable genes rplO map, rps19 rps3, cpn60, tuf, pyrH frr, hflB were amplified, sequenced and analyzed. Additional molecular characteristic of 16S rDNA was carried out by the restriction fragment lenght polymorhism.
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