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Determination of the concentration of alpha-1-antitrypsin in the stool by immunoassay method
Plačková, Marie ; Kocna, Petr (advisor) ; Průša, Richard (referee)
Determination of the concentration of α1 - antitrypsin in the stool is a diagnostic indicator of inflammatory diseases of the small and the large intestine, especially malabsorption syndrome. α1 - antitrypsin belongs to the family of plasma proteins with antiproteinase effect. α1 - antitrypsin is synthesized in liver, in small amount in macrophage and is a protease inhibitor of serine proteases sercected from neutrophils. α1 - antitrypsin is acute phase protein. Higher α1 - antitrypsin values are in early phase of inflammation associated with raised CRP and other pozitive acute phase proteins. Fecal α1 - antitrypsin clearance is a sensitive and specific marker of protein loss. For α1 - antitrypsin determination in stool samples ELISA method can be used. ELISA is noncompetetive immunoassay used to detect presence of antibody or an antigen in a sample. The aim of this work was to compare two ELISA sets (Immundiagnostik and Ridascreen) used for determination α1 - antitrypsin in the stool. Then examine stability of α1 - antitrypsin in the stool and in extract prepared from stool in various storing conditions temperature and time. After this establish this method as routine in laboratory. 20 patient stool samples were examined to compare ELISA sets. Samples were suggested to be α1 - antitrypsin...
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Common spoken language in Blížkovice
PLAČKOVÁ, Marie
The aim of this dialectological thesis is to find what distinctive features are there in the common language of speakers coming from the region Blížkovice. The research was conducted on the basis of speech recordings of people of three generations (the oldest, elder and the youngest one). The work also includes a general description of the subgroup centralmoravian south. Special attention is given to phonological, formative and lexical features of the region.
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The Hospital Spiritual Care. Existing Practice, Experience and Expectations Illustrated by Examples of Chosen Prague Hospitals
Plačková, Marie ; Beneš, Ladislav (advisor) ; Baštecká, Bohumila (referee)
The main goal of this diploma thesis is to reflect the hospital spiritual care in Czech Republic nowadays and to compare it with its beginnings. The theoretical part maintains holistic approach to human being and emphasizes spiritual needs as a basis for hospital spiritual care. It also gives an overview of recent forms of hospital spiritual care in Czech Republic. The research part examines the patients' opinions and experience of spiritual care during their stay in hospitals. Questionnaire-based survey was done in seven Prague hospitals with 237 patients (96 of them used the chaplain service, 141 did not). The short interviews with Prague chaplains and patients regarding spiritual care complete the work. Research findings result in non-answered (and maybe non-answerable) questions: Can hospital chaplains quit their church membership and affiliation and not to serve to the church evangelization Mission? Is it possible to be "a spiritual, but non religious" chaplain and not to lose the chaplain identity? Is it - in general - possible to distinguish "spirituality" from "religiosity"?
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Determination of the concentration of alpha-1-antitrypsin in the stool by immunoassay method
Plačková, Marie ; Kocna, Petr (advisor) ; Průša, Richard (referee)
Determination of the concentration of α1 - antitrypsin in the stool is a diagnostic indicator of inflammatory diseases of the small and the large intestine, especially malabsorption syndrome. α1 - antitrypsin belongs to the family of plasma proteins with antiproteinase effect. α1 - antitrypsin is synthesized in liver, in small amount in macrophage and is a protease inhibitor of serine proteases sercected from neutrophils. α1 - antitrypsin is acute phase protein. Higher α1 - antitrypsin values are in early phase of inflammation associated with raised CRP and other pozitive acute phase proteins. Fecal α1 - antitrypsin clearance is a sensitive and specific marker of protein loss. For α1 - antitrypsin determination in stool samples ELISA method can be used. ELISA is noncompetetive immunoassay used to detect presence of antibody or an antigen in a sample. The aim of this work was to compare two ELISA sets (Immundiagnostik and Ridascreen) used for determination α1 - antitrypsin in the stool. Then examine stability of α1 - antitrypsin in the stool and in extract prepared from stool in various storing conditions temperature and time. After this establish this method as routine in laboratory. 20 patient stool samples were examined to compare ELISA sets. Samples were suggested to be α1 - antitrypsin...
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To a language and style of regional weekly newspaper "Znojemský týden"
PLAČKOVÁ, Marie
The aim of a bachelor?s work on a topic "To a language and a style of a regional weekly newspaper Znojemský týden" is a complex stylistic analysis of weekly newspaper Znojemský týden for the whole year 2011. The work is divided into two parts - a theoretical part and a practical part. The theoretical part deals with journalistic functional style ? its characteristics, history and an internal differentiation and also with subtitles and expressional means which are typical for journalistic functional style. The practical part covers partly the characteristics of Znojemský týden and partly language analysis on a phonetic, morphological, syntactical and lexical level. Expressional means are characterized here and there are examples mentioned from Znojemský týden. The biggest attention is devoted to a lexical level.
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