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Morphological characteristics of steatosis development in precision cut liver slices and the possibility of its modification
 Přibáňová, Petra ; Němečková, Ivana (advisor) ; Nachtigal, Petr (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Biological and Medical Sciences Study program: Laboratory diagnostics in healthcare Author: Petra Přibáňová Supervisor: RNDr. Ivana Němečková, Ph.D. Title of the thesis: Morphological characteristics of steatosis development in precision cut liver slices and the possibility of its modification Background and the aim of the thesis: Non-alcoholic hepatic steatosis is one of the most common causes of chronic liver disease. It is considered to be a manifestation of a metabolic syndrome associated with obesity, dyslipidaemia and type II diabetes mellitus. The aim of the bachelor thesis was to describe the anatomy and histology of the liver and hepatic steatosis, as well as to prepare histological slides, their subsequent staining and observation of the influence of different substances on the development of steatosis in liver tissue. Methods: Mouse liver was used to prepare precision cut liver slices (PCLS). The slices were then fixed and incubated in different media. Paraffine slides stained with hematoxylin- eosin or oil red were observed under a microscope. Results and conclusion: When comparing control PCLS without incubation with slices incubated for 36 hours in basic Williams' medium, we found that necrosis...
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