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Control the molecular weight of hyaluronic acid (HA) by adjusting the specific growth rate of culture Streptococcus equi subsp. zooepidemicus
Osičková, Jana ; Pepeliaev,, Stanislav (referee) ; Franke,, Lukáš (advisor)
This master thesis focuses on the effect of cultivation parameters on hyaluronic acid synthesis during its biotechnological production. The cultivation parameters were temperature of the cultivation, aeration, agitation, carbon source and addition of phosphatidylcholine. Changes in molecular weight and yield of hyaluronic acid, growth of biomass and medium viscosity were observed. From the obtained data we learned, that the specific growth rate greatly impacts final characteristics of hyaluronic acid. Under suboptimal temperatures a high molecular weight polymer was synthesised along with comparable yields from the control cultivation. High temperatures (40 °C) caused a dip in molecular weight. The next cultivation parameters were aeration and agitation. The highest molecular weights were obtained in cultivations with high agitation rates and intensive aeration, specifically 1 vvm/800 rpm and 2 vvm/800 rpm. Agitation had a bigger influence on molecular weight than aeration. When the carbon source was changed from sucrose to glucose, the lowest molecular weight and yield were obtained. Addition of phosphatidylcholine with concentration 160 mg/l had a positive effect on the cultivation, where the molecular weight grew and biomass growth was higher.
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Změny v chování spotřebitelů při nákupu potravin a nealkoholických nápojů
Osičková, Jana
The bachelor thesis deals with changes in consumer behaviour when buying food and non-alcoholic beverages. The aim of the bachelor thesis is to identify factors that influence consumer behaviour when buying food and non-alcoholic beverages, identify what factors are influencing consumers today, compare the resulting data with historical studies that have been conducted in this area and propose recommendations for Czech retailers in the food and non-alcoholic beverage market. The data were obtained using quantitative research and were processed in Microsoft Excel and Statistica. The results from the questionnaire survey showed that the factors of necessity, quality and previous experience have the greatest influence on consumers.
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Control the molecular weight of hyaluronic acid (HA) by adjusting the specific growth rate of culture Streptococcus equi subsp. zooepidemicus
Osičková, Jana ; Pepeliaev,, Stanislav (referee) ; Franke,, Lukáš (advisor)
This master thesis focuses on the effect of cultivation parameters on hyaluronic acid synthesis during its biotechnological production. The cultivation parameters were temperature of the cultivation, aeration, agitation, carbon source and addition of phosphatidylcholine. Changes in molecular weight and yield of hyaluronic acid, growth of biomass and medium viscosity were observed. From the obtained data we learned, that the specific growth rate greatly impacts final characteristics of hyaluronic acid. Under suboptimal temperatures a high molecular weight polymer was synthesised along with comparable yields from the control cultivation. High temperatures (40 °C) caused a dip in molecular weight. The next cultivation parameters were aeration and agitation. The highest molecular weights were obtained in cultivations with high agitation rates and intensive aeration, specifically 1 vvm/800 rpm and 2 vvm/800 rpm. Agitation had a bigger influence on molecular weight than aeration. When the carbon source was changed from sucrose to glucose, the lowest molecular weight and yield were obtained. Addition of phosphatidylcholine with concentration 160 mg/l had a positive effect on the cultivation, where the molecular weight grew and biomass growth was higher.
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