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Effect of camphor isomers on the expression of drug-metabolizing enzymes in human liver cells
Ondřejová, Michaela ; Boušová, Iva (advisor) ; Ambrož, Martin (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Michaela Smolíková, DiS. Supervisor: doc. PharmDr. Iva Boušová, Ph.D. Advisor: Mgr. Michaela Šadibolová Title of diploma thesis: Effect of camphor isomers on the expression of drug-metabolizing enzymes in human liver cells Camphor is a cyclic ketone from the group of monoterpenes. Two camphor isomers occur in nature. Currently, camphor is used as an antiseptic, analgesic, rubefacients, and anti- inflammatorydrug. Camphor is sed mainly in preparations for topical use, where it has a mild anesthetic effect and a feelingof warmth together with its characteristicallystrong fresh scent. Most camphor intoxications are caused by improper use or dosing. The aim of the diploma thesis was to determine the effect of isomers (+)-camphor and (-)-camphor on the expression of selected phase I and II biotransformation enzymes. The precision-cut human liver slices prepared from a total of three patients, two men and one woman at the age range of 46 - 49 years,were used as biological material.Liver slices were incubatedwith10 µM and 50 µM (+)- camphor and (-)-camphor for 24hours at 37řC. Proteinexpressionof phase I (cytochrome P450 (CYP) 3A4, CYP2C, aldo-keto reductase (AKR) 1C3), and phase II enzymes...
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