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Regeneration initiation of Xenopus laevis tail-functional study
Netušil, Jiří ; Šindelka, Radek (advisor) ; Petr, Jaroslav (referee)
The recent introduction of high-throughput sequencing techniques rapidly changes our perception of vertebrate regeneration. One of the model organisms intensely studied for its regenerative potential is the embryonic tadpole stage of African clawed frog (Xenopus laevis). This thesis aims to review and functionally validate some of the latest findings gained by RNA sequencing of regenerating Xenopus laevis tail. We specifically focus on gene expression changes during the early phases of regeneration and how their absence affects the progression and phenotypic outcome of this process. Our analysis confirms that Regeneration initiating cells (RICs) identified by single-cell and spatial RNA sequencing represent a vital element in successful tail regeneration. The RIC marker genes mmp9 and pmepa1 affect the processes linked to extracellular matrix remodelling and the migration pattern of previously described Regeneration organizing cells (ROCs) while not interfering with the myeloid cell lineage. We further demonstrate a novel use of Vivo Morpholino oligonucleotides in a transient knockdown assay and offer an assessment of its benefits and limitations. This thesis highlights the importance of spatio-temporal regulation happening on both cellular and molecular levels during the initial 24 hours of...
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Gene expression differences during regeneration in model organisms
Netušil, Jiří ; Šindelka, Radek (advisor) ; Hason, Martina (referee)
With new progressive methods allowing us to study natural regeneration in model organisms, we have an opportunity to gain important insights into the very essence of this process. These insights might help us radically improve the current state of therapeutic approaches based on tissue replacement. Many different animal models display an incredible ability to restore various body parts, allowing them to escape predators and avoid premature death. While invertebrate models give us a chance to investigate the fundamental elements of regeneration, vertebrates represent systems often more resembling human biology. This thesis outlines the variability of regeneration in frequently studied model organisms with a special emphasis on the impact of gene expression. Keywords: regeneration, model organism, gene expression, injury
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Benefit of creditor in civil proceedings
Netušil, Jiří ; Pohl, Tomáš (advisor) ; Smolík, Petr (referee)
Benefit of creditor in civil proceedings is the topic of the thesis. The benefit of creditor is defined for the purposes of this thesis as a complex of three conditioning factors - the strictness of legislation, the representation of creditor by a lawyer and the passivity of the defendant debtor. Selected institutes of civil proceedings are then measured against the principle of equality of the parties as a fundamental principle of the civil procedure. The thesis, which has introductory and closing chapter too, is divided into five chapters, whose aim is the analysis of civil procedure and its various institutes in terms of potential, but also the real benefit of the creditor as plaintiff. The rigorosum work also includes selected institutes in the first phase of the civil procedure, which is the main part of the work, then deals with selected questions of notarial record with consent to execute, an arbitration procedure, execution and insolvency proceedings.
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Creation and termination of membership in cooperative
Netušil, Jiří ; Oehm, Jaroslav (advisor) ; Liška, Petr (referee)
CREATION AND TERMINATION OF MEMBERSHIP IN COOPERATIVE Summary The theme of the thesis is Creation and termination of membership in cooperative. Cooperative as a legal entity is one type of an association of people for some specific purpose which is characterized by them. Any cooperative cannot be without its members. Therefore the legal amendment of membership's creation and termination in cooperative creates very important legal amendment part in cooperatives. The thesis, which has introductory and closing chapter too, is divided into five chapters which are arranged by way, whose goal is to give a systematic explanation of creation and termination of membership in cooperative in the Czech Republic. The thesis includes relevant decisions of the Czech courts and opinions from books and articles. The introductory chapter contains the main purposes of the thesis. The first and second chapters are a common introduction to the law of cooperatives. The first chapter provides a brief historical law sources explanation of cooperatives and valid legal amendment of cooperatives. It pays attention on a bill which includes a new legal amendment of cooperatives. The comparison between Czech and German legal cooperatives amendments is a part of this chapter too. The second chapter explains basic terms which are...
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