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Analytical Properties of Selected Phytocannabinoids
Myšíková, Jitka ; Jezberová, Michaela (advisor) ; Kubíček, Vladimír (referee)
( English version ) Title: Analytical Properties of Selected Phytocannabinoids Author: Jitka Myšíková Supervisor: RNDr. Michaela Hamerníková, Ph.D. Department: Department of Biophysics and Physical Chemistry I investigated the issue how to determine the delta-9-tetrahydrocannabinol (∆9 -THC) and tetrahydrocannabinol acids (THCAs) by HPLC and GC. THCAs, the precursors of ∆9 - THC, are the compounds of Cannabis sativa. They are unstable, decarboxylate easily and convert into ∆9 -THC at higher temperature. I examined the THCAs instability both in marihuana and in extracts from marihuana at the higher temperature. During my experiments the temperature conversion of THCA into ∆9 -THC was the highest at temperatures of approx. 100C-120C and the ∆9 -THC content decreased at temperatures over 120C. I evaluated the influence of various stimuli (e.g. light, UV radiation, boiling of marihuana in n-hexane, various temperatures, ways of storage) on the ∆9 -THC content in marihuana both during drying and preparation of samples before the analysis. The increased ∆9 -THC content was not only the result of higher temperature but also the UV radiation, however, to a smaller extent. The influence of the UV radiation should be further investigated. I compared the values of the ∆9 -THC content determinated by HPLC...
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Analytical Properties of Selected Phytocannabinoids
Myšíková, Jitka ; Jezberová, Michaela (advisor) ; Kubíček, Vladimír (referee)
( English version ) Title: Analytical Properties of Selected Phytocannabinoids Author: Jitka Myšíková Supervisor: RNDr. Michaela Hamerníková, Ph.D. Department: Department of Biophysics and Physical Chemistry I investigated the issue how to determine the delta-9-tetrahydrocannabinol (∆9 -THC) and tetrahydrocannabinol acids (THCAs) by HPLC and GC. THCAs, the precursors of ∆9 - THC, are the compounds of Cannabis sativa. They are unstable, decarboxylate easily and convert into ∆9 -THC at higher temperature. I examined the THCAs instability both in marihuana and in extracts from marihuana at the higher temperature. During my experiments the temperature conversion of THCA into ∆9 -THC was the highest at temperatures of approx. 100C-120C and the ∆9 -THC content decreased at temperatures over 120C. I evaluated the influence of various stimuli (e.g. light, UV radiation, boiling of marihuana in n-hexane, various temperatures, ways of storage) on the ∆9 -THC content in marihuana both during drying and preparation of samples before the analysis. The increased ∆9 -THC content was not only the result of higher temperature but also the UV radiation, however, to a smaller extent. The influence of the UV radiation should be further investigated. I compared the values of the ∆9 -THC content determinated by HPLC...
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Stability of phytocanabinoids during storage of Cannabis sativa
Myšíková, Jitka ; Jezberová, Michaela (advisor) ; Mokrý, Milan (referee)
(English version) Title: Stability of phytocanabinoids during storage of Cannabis sativa Author: Mgr. Jitka Myšíková Supervisor: RNDr. Michaela Hamerníková, Ph.D. The rigorous work deals with the problems of determination of ∆9 -THC, CBD and CBN and their precursors. It assesses the influence of storage time and way on marijuana and on n-hexan extract of marijuana. A validation of the method of ∆9 -THC, CBD and CBN determination was carried out through HPLC with UV detection at wave length of 220 nm, mobile phase methanol : water ( 85 : 15), colomn SUPELCOSILTM LC-18-DB. The main aim was to detect the influence of various storage ways and storage time on principal content items of marijuana. There were 2 samples of marijuana available, each of them was divided in 12 parts, whereas 6 parts were placed in a plastic bag and the other 6 in a linen bag. After that one part of the sample in the plastic bag and one in the linen bag were located in 6 places, respectively from each sample. The places of storage were choosen with respect to possible location by marijuana users or to their possible experiments with this drug. The observation went on for 8,5 months. The samples were put in a refrigerator, an ice-box, a room with daylight, on an exterior window sill, under a UV lamp or a sample was soaked and...
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