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Rating of blood cholinesterase activities in the population of Central Europe.
 Maděryčová, Zuzana ; Šepsová, Vendula (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of: Pharmacology and Toxicology Candidate: Mgr. Zuzana Maděryčová Consultant: PharmDr. Marie Vopršalová, CSc. Specialized consultant: PharmDr. Jana Žďárová Karasová, Ph.D. Title of Thesis: Rating of blood cholinesterase activities in the population of Central Europe This study is focused on the assessment of the average value of acetylcholinesterase (AChE, EC 3.1.1.7) and butyrylcholinesterase (BChE, EC 3.1.1.8) activity in blood and plasma samples of healthy donors (n = 387). Spectrophotometric determination of blood cholinesterase activities was based on the Ellman's reaction, the reaction course of which was adjusted according to the character of the biological material (Worek et al. 1999). The impact of sex, age and smoking on blood cholinesterase activities was observed in a representative sample of the target population. Data were related to factors affecting the result of the measurement such as haemoglobin (Hb) concentration in the case of AChE activity assessment and environment temperature during the measurement. Statistical data evaluation provided complex information about blood cholinesterase activities in the healthy population. Results of this work contribute considerably to diagnostics of organophosphate...
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