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Small fiber neuropathy in peripheral and autonomic nervous system in patients with diabetes
Potočková, Veronika ; Mazanec, Radim (advisor) ; Voháňka, Stanislav (referee) ; Kunc, Pavel (referee)
Small fiber neuropathy in peripheral and autonomic nervous system in patients with diabetes Abstract This dissertation thesis wants to draw attention to the relevance of early detection of small nerve fibers damage in the somatic and autonomic nervous systems in patients with type 1 and type 2 diabetes mellitus (T1DM, T2DM). Only early diagnosis can prevent the severe complications that progressive forms of diabetic neuropathy (DN) bring. The main objectives of this work were to evaluate the possibility of using thermal quantitative sensory testing (TQST) to identify patients with diabetes at higher risk of cardiac autonomic neuropathy (CAN) and distal symmetric polyneuropathy (DSPN) with predominant small nerve fibers impairment. Other aims were to assess the impact of CAN on the development of carotid atherosclerosis and to determine the prevalence and risk factors for depression and anxiety in diabetic patients with and without DN. The results show that TQST can be used as a screening tool to identify patients with a higher risk of CAN. We demonstrated a significant effect of CAN presence on carotid intima-media thickness in patients with T1DM, independently of known risk factors of atherosclerosis. Such a finding suggests that CAN may play a role in the pathogenesis of carotid atherosclerosis. Another...
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Neuroregeneration after spinal cord injury and in amyotrophic lateral sclerosis -possibilities for stem cell therapy
Forostyak, Serhiy ; Syková, Eva (advisor) ; Rokyta, Richard (referee) ; Mazanec, Radim (referee)
Adipose-derived MSC could be used as an alternative for bone marrow MSC in the treatment of acute SCI. We used the intraspinal grafting of rat adipose-derived naïve and predifferentiated MSC to improve motor function after a balloon-induced compression lesion of the rat spinal cord. Grafted cells survived for seven weeks after transplantation, improved motor activity and integrated into the host tissue. They expressed the oligodenrocyte precursor marker NG2 and, occasionally, the astrocytic marker GFAP, but did not transdifferentiate into a neuronal phenotype. Bone marrow MSC may change the disease course and extend lifespan in a rat model of ALS. Combined intraspinal and intravenous transplantation of rat BMSC was performed in symptomatic rats overexpressing the SOD1 G93A gene. Cell-treated animals lived longer compared with sham-treated rats and displayed significantly improved motor activity and grip strength. Rat BMSC survived until the end stage of the disease and were migrating along the white matter of the spinal cord. Grafted cells increased the number of host cells displaying positive staining for neurofilaments and significantly increased the number and also the size of the remaining spinal motoneurons 10-11 weeks after delivery, compared with vehicle-injection. The defragmentation of DNA, a sign...
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Neuroregeneration after spinal cord injury and in amyotrophic lateral sclerosis -possibilities for stem cell therapy
Forostyak, Serhiy ; Syková, Eva (advisor) ; Rokyta, Richard (referee) ; Mazanec, Radim (referee)
Adipose-derived MSC could be used as an alternative for bone marrow MSC in the treatment of acute SCI. We used the intraspinal grafting of rat adipose-derived naïve and predifferentiated MSC to improve motor function after a balloon-induced compression lesion of the rat spinal cord. Grafted cells survived for seven weeks after transplantation, improved motor activity and integrated into the host tissue. They expressed the oligodenrocyte precursor marker NG2 and, occasionally, the astrocytic marker GFAP, but did not transdifferentiate into a neuronal phenotype. Bone marrow MSC may change the disease course and extend lifespan in a rat model of ALS. Combined intraspinal and intravenous transplantation of rat BMSC was performed in symptomatic rats overexpressing the SOD1 G93A gene. Cell-treated animals lived longer compared with sham-treated rats and displayed significantly improved motor activity and grip strength. Rat BMSC survived until the end stage of the disease and were migrating along the white matter of the spinal cord. Grafted cells increased the number of host cells displaying positive staining for neurofilaments and significantly increased the number and also the size of the remaining spinal motoneurons 10-11 weeks after delivery, compared with vehicle-injection. The defragmentation of DNA, a sign...
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The comparison of physics textbooks for basic schools
MAZANEC, Radim
The thesis deals with the comparison of physics textbooks for primary schools. The purpose of this thesis is to determine the representation of individual sets of textbooks in primary schools in the South Bohemian Region, to develop and test custom teaching concepts revolving around thematic unit of 'Simple Machines'. This work is addressed primarily to teachers as a tool to enhance teaching effectiveness, to support the imagination and to increase interest in physics among pupils. These are the reasons why thematic unit, called Simple Machines, has been developed in form of interactive presentation, whose contribution and efficiency has been practically verified directly during physics classes in few selected primary schools. Benefits have been verified through accredited didactic tests and their evaluation and results are processed at the end of this thesis.
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