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Characterization of liposomal formulations for brain-targeting.
Labajová, Andrea ; Šklubalová, Zdeňka (advisor) ; Zbytovská, Jarmila (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of: Department of Pharmaceutical Technology Consultant: Assoc. Prof. Zdeňka Šklubalová, Ph.D. Robin Tremmel, Ph.D. student Student: Andrea Labajová Title of Thesis: Characterization of liposomal formulations for brain- targeting This paper deals with characterization of liposomal formulations with encapsulated triethylenetetramine (TETA), which is selective CuII - chelator used in the treatment of Wilson's disease for decades. Liposomal formulations were prepared by a film hydration method with subsequent dual asymmetric centrifugation with the addition of 2.5 mol/l TETA solution dissolved at pH3 or/and pH7, respectively. Size exclusion chromatography (SEC) was performed to separate free-TETA from the encapsulated-one at day 1, 2, 3, 4, 5 and 8. Two methods of liposome purification, the one column method and the two columns method, were used. The size and the size distribution of prepared liposomes were measured by photon correlation spectroscopy (PCS) at each day of storage. The concentration of encapsulated TETA, as well as the concentrations of cholesterol before the SEC and after the SEC were determined by HPLC in order to express the encapsulation efficiency. No influence of pH or the method of purification on the...
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