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God's Unity and God's Relational Character in the Thinking and Biblical Interpretation of Philo Judaeus
LANDOVÁ, Barbora
The thesis analyzes how the Hellenistic Jewish philosopher Philo of Alexandria deals with passages in the Bible where God experiences any kind of human emotions. It aims to present the two sides of Philo's persona - the Greek and the Jewish. These two worlds (the world of philosophy and that of the Bible) are constituent for Philo's thinking, however, they also provide a distinct rendering of God. The aim is to ascertain how Philo deals with passages in the Bible where God seems to have a changeable character while holding a strong philosophical concept of him. The thesis adheres to the hypothesis that his depiction of God is bound to the literary genre he applies when rendering God. It takes into consideration Philo's Quod Deus sit Immutabilis, Quaestiones et Solutiones in Genesin et Exodum and De Vita Mosis while differentiating between their distinct literary genres.
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Structural studies of an abasic site DNA damage repair and DNA interstrand cross-link formation
Landová, Barbora
DNA damage refers to any alteration or modification in the DNA structure that deviates from its natural state. Abasic site (Ap site) is one of the most common DNA lesions resulting from spontaneous depurination/depyrimidination or enzymatic base excision. When left unrepaired it can lead to a cascade of genetic mutations, potentially causing diseases like cancer. Understanding DNA repair mechanisms is vital for medical research and applications. Bacterial MutM is a DNA repair glycosylase, removing DNA damage generated by oxidative stress and preventing mutations and genomic instability. MutM belongs to the Fpg/Nei family of procaryotic enzymes, sharing structural and functional similarities with their eukaryotic counterparts, such as NEIL1-NEIL3. Here, I present two crystal structures of MutM from pathogenic Neisseria meningitidis: MutM holoenzyme and MutM bound to DNA. The free enzyme exists in an open conformation, while upon binding to DNA, both the enzyme and DNA undergo substantial structural changes and domain rearrangement. One of the DNA lesion repaired by MutM is the Ap site, which, if not repaired, may spontaneously lead to the formation of an abasic site interstrand crosslink (Ap-ICL) with an adjacent adenine in the opposite strand. NEIL3 glycosylase is known to remove Ap-ICL. With a...
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Structural studies of an abasic site DNA damage repair and DNA interstrand cross-link formation
Landová, Barbora ; Bouřa, Evžen (advisor) ; Bařinka, Cyril (referee) ; Schneider, Bohdan (referee)
DNA damage refers to any alteration or modification in the DNA structure that deviates from its natural state. Abasic site (Ap site) is one of the most common DNA lesions resulting from spontaneous depurination/depyrimidination or enzymatic base excision. When left unrepaired it can lead to a cascade of genetic mutations, potentially causing diseases like cancer. Understanding DNA repair mechanisms is vital for medical research and applications. Bacterial MutM is a DNA repair glycosylase, removing DNA damage generated by oxidative stress and preventing mutations and genomic instability. MutM belongs to the Fpg/Nei family of procaryotic enzymes, sharing structural and functional similarities with their eukaryotic counterparts, such as NEIL1-NEIL3. Here, I present two crystal structures of MutM from pathogenic Neisseria meningitidis: MutM holoenzyme and MutM bound to DNA. The free enzyme exists in an open conformation, while upon binding to DNA, both the enzyme and DNA undergo substantial structural changes and domain rearrangement. One of the DNA lesion repaired by MutM is the Ap site, which, if not repaired, may spontaneously lead to the formation of an abasic site interstrand crosslink (Ap-ICL) with an adjacent adenine in the opposite strand. NEIL3 glycosylase is known to remove Ap-ICL. With a...
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Election of Israel in Kuzari by Judah Halevi
LANDOVÁ, Barbora
The thesis deals with conception of the teaching of the election of Israel in a work called Kuzari by the medieval Jewish philosopher Judah Halevi. In the third chapter, it is focused on Halevi's conception of the election of people, land, and language. In the fourth chapter, there is a comparison of the interpretation of Ex 19,5 by standard commentaries with Halevi's own in a connection with the election of Israel.
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Structure and molecular mechanisms of DNA repair by Nei glycosylase
Landová, Barbora ; Šilhán, Jan (advisor) ; Lux, Vanda (referee)
Abasic sites (Ap site, from apurinic/apyrimidinic) are one of the most common lesions generated in DNA by spontaneous base loss or DNA repair processes. There are two equilibrating forms of an Ap site - ring-open aldehyde and cyclic hemiacetal. Ring- opened aldehydes are reactive electrophilic groups capable of formation covalent adduct with nucleophilic sites in DNA. DNA interstrand cross-link (ICL) resulting from the Ap sites is formed spontaneously as a covalent bond between ring-open aldehyde and amin group of adenin residue in the opposite strand of double stranded DNA. ICLs block DNA replication and transcription. The formation of Ap site derived ICL is relatively long process taking several hours. We assume that the ring-opening of an abasic site is the rate-limiting step in the formation of the thermodynamic ICL. However, formation, stability and DNA repair of Ap-ICL are still poorly understood processes. Here, I have set up mechanistic in vitro experiments to reveal and calculate the probability of Ap-ICl formation in vivo. In more detail, I study the rates of formation of Ap-ICLs in the sequence context of neighbouring nucleotides of freshly formed covalent bond of ICL. I focus on sequence preference, the influence of AT/ GC rich regions and the length of oligonucleotides. I have...
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Preparation of expression construct and purification of xNEIL3 glycosylase
Landová, Barbora ; Obšil, Tomáš (advisor) ; Hlouchová, Klára (referee)
DNA glycosylases are important enzymes responsible for the recognition and removal of base DNA damage. These DNA lesions are formed spontaneously or as an effect of environmental agents. NEIL3 (Endonuklease VIII - like 3) enzyme belongs to a Fpg/Nei family of glycosylases implicated in the DNA repair of oxidative bases, abasic sites. Recently, it has been shown that these enzymes are capable of removal of one type of interstrand crosslink by so unknown repair pathway. Unrepaired DNA crosslinks block DNA replication and may lead to cell death or sever chromosomal damage and later even cancer. This thesis is focused on revealing molecular mechanisms of the interstrand DNA- DNA cross-link repair by NEIL3 glycosylase. (In Czech)
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Dance Movement Therapy
Landová, Barbora ; Sivek, Vojtěch (advisor) ; Špinarová, Jana (referee)
It is a nonverbal expressive psychotherapeutic method based on work with a body, space, energy, strength, time and at last on music. It is not focused on an aesthetic feature of dance and movement, on the other hand it is oriented to expressive, creative, and an individual potential of any personality. The subject matter of my work is to show theoretic information connected with a dance movement therapy included historical development, its methods, principles and rules. The term dance movement therapy is in an intimately connection with psychotherapy, that could be shown in the first chapter. There are details of theoretical approaches of individual and group clients' work described in other chapters too. You can get to know about my practical experiences learned in a course of dance movement therapy called "Elemental express" based on my theoretic information. Powered by TCPDF (www.tcpdf.org)
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